Preparation of a Biomimetic Poly-Phosphatidylserine Monolithic Column for Immobilized Artificial Membrane Chromatography

IF 2.8 3区工程技术 Q2 CHEMISTRY, ANALYTICAL

Journal of separation science Pub Date : 2025-03-20 DOI:10.1002/jssc.70120

Xueyan Zhu, Xianglong Zhao, Zhongkang Wang, Xiaoyuan Xie, Jincai Wang, Jianlong Liang, Zheng Liu, Tao Deng, Bin Yang, Jialiang Guo

{"title":"Preparation of a Biomimetic Poly-Phosphatidylserine Monolithic Column for Immobilized Artificial Membrane Chromatography","authors":"Xueyan Zhu, Xianglong Zhao, Zhongkang Wang, Xiaoyuan Xie, Jincai Wang, Jianlong Liang, Zheng Liu, Tao Deng, Bin Yang, Jialiang Guo","doi":"10.1002/jssc.70120","DOIUrl":null,"url":null,"abstract":"<div>\n \n The pharmacokinetic properties of drugs are significantly influenced by their interactions with cell membranes. Phosphatidylserine (PS) as one of the crucial membrane phospholipids is present abundantly in biological cells. In this study, a PS-mimicking phospholipid membrane polymer monolithic column was developed using the synthetic compound 2-methylacryloxydodecyl phosphatidylserine (MDPS). The physicochemical properties and chromatographic performance of the poly(MDPS-co-ethylene dimethacrylate (EDMA)) monolithic column were evaluated through scanning electron microscopy (SEM) and nano-high-performance liquid chromatography (HPLC). The retention mechanism was investigated by eluting the acidic, neutral, and basic compounds under the same gradient conditions, and the performance of the poly(MDPS-co-EDMA) monolithic column was compared with that of a poly(12-methacryloyl n-dodecylphosphocholine (MDPC)-co-EDMA) monolithic column. The poly(MDPS-co-EDMA) column demonstrated effective separation capabilities for small peptides, protein enzymatic hydrolysis polypeptides, and pharmaceutical compounds. Furthermore, the column was also employed to predict the blood–brain barrier (BBB) permeability of drugs, and the correlation coefficient of 0.73 indicates the promising potential of poly(MDPS-co-EDMA) monolithic column for predicting the ability of drugs to cross the BBB.\n </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of separation science","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jssc.70120","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

The pharmacokinetic properties of drugs are significantly influenced by their interactions with cell membranes. Phosphatidylserine (PS) as one of the crucial membrane phospholipids is present abundantly in biological cells. In this study, a PS-mimicking phospholipid membrane polymer monolithic column was developed using the synthetic compound 2-methylacryloxydodecyl phosphatidylserine (MDPS). The physicochemical properties and chromatographic performance of the poly(MDPS-co-ethylene dimethacrylate (EDMA)) monolithic column were evaluated through scanning electron microscopy (SEM) and nano-high-performance liquid chromatography (HPLC). The retention mechanism was investigated by eluting the acidic, neutral, and basic compounds under the same gradient conditions, and the performance of the poly(MDPS-co-EDMA) monolithic column was compared with that of a poly(12-methacryloyl n-dodecylphosphocholine (MDPC)-co-EDMA) monolithic column. The poly(MDPS-co-EDMA) column demonstrated effective separation capabilities for small peptides, protein enzymatic hydrolysis polypeptides, and pharmaceutical compounds. Furthermore, the column was also employed to predict the blood–brain barrier (BBB) permeability of drugs, and the correlation coefficient of 0.73 indicates the promising potential of poly(MDPS-co-EDMA) monolithic column for predicting the ability of drugs to cross the BBB.

查看原文本刊更多论文

制备用于固定人工膜层析的仿生物聚磷脂酰丝氨酸整体柱。

药物的药代动力学性质受其与细胞膜的相互作用的显著影响。磷脂酰丝氨酸（PS）作为重要的膜磷脂之一，在生物细胞中大量存在。本研究以合成化合物2-甲基丙烯氧基十二烷基磷脂酰丝氨酸（MDPS）为原料，制备了一种模拟ps的磷脂膜聚合物整体柱。采用扫描电镜（SEM）和纳米高效液相色谱（HPLC）对聚mdps -二甲基丙烯酸乙酯（EDMA）整体柱的理化性质和色谱性能进行了评价。通过在相同梯度条件下洗脱酸性、中性和碱性化合物，考察了其保留机理，并比较了聚（MDPS-co-EDMA）整体柱与聚（12-甲基丙烯酰n-十二烷基磷酸胆碱(MDPC)-co-EDMA）整体柱的性能。聚（MDPS-co-EDMA）色谱柱对小肽、蛋白酶解多肽和药物化合物具有有效的分离能力。此外，该柱还可用于预测药物的血脑屏障通透性，相关系数为0.73，表明poly（MDPS-co-EDMA）整体柱在预测药物通过血脑屏障能力方面具有广阔的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of separation science 化学-分析化学

CiteScore

6.30

自引率

16.10%

发文量

408

审稿时长

1.8 months

期刊介绍： The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.