Live imaging in zebrafish reveals tissue-specific strategies for amoeboid migration.

IF 3.7 2区生物学 Q1 DEVELOPMENTAL BIOLOGY

Development Pub Date : 2025-03-21 DOI:10.1242/dev.204351

Tanner F Robertson, Jon Schrope, Zoe Zwick, Julie Rindy, Adam Horn, Yiran Hou, Anna Huttenlocher

引用次数: 0

Abstract

Amoeboid cells like leukocytes can enter and migrate in diverse tissues, even though tissues vary widely in their chemical and mechanical composition. Here, we imaged motile T cells as they colonized peripheral tissues during zebrafish development to ask if cells tailor their migration strategy to their local tissue environment. We found that T cells in most sites migrated with F-actin-rich leading-edge pseudopods, matching how they migrate in vitro. T cells notably deviated from this strategy in the epidermis, where they instead migrated using a rearward concentration of F-actin and stable leading-edge blebs. This mode of migration occurs under planar confinement in vitro, and we found the stratified keratinocyte layers of the epidermis also impose planar-like confinement on leukocytes in vivo. Collectively, our data indicate that immune cells adapt their migration strategy to navigate different tissue geometries in vivo.

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斑马鱼的实时成像揭示了变形虫迁移的组织特异性策略。

像白细胞这样的变形虫细胞可以在不同的组织中进入和迁移，即使组织在化学和机械组成上有很大的不同。在这里，我们对斑马鱼发育过程中运动T细胞定殖在外周组织的过程进行了成像，以了解细胞是否根据其局部组织环境调整其迁移策略。我们发现，大多数部位的T细胞与富含f -肌动蛋白的前沿假足一起迁移，这与它们在体外的迁移方式相匹配。T细胞在表皮中明显偏离了这一策略，而是利用f -肌动蛋白的后向浓度和稳定的前沿气泡进行迁移。这种迁移模式发生在体外的平面约束下，我们发现表皮的分层角质形成细胞层也对体内的白细胞施加平面约束。总的来说，我们的数据表明免疫细胞在体内调整其迁移策略以导航不同的组织几何形状。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Development 生物-发育生物学

CiteScore

6.70

自引率

4.30%

发文量

433

审稿时长

3 months

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