Advances in CRISPR-enabled genome-wide screens in yeast.

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

FEMS yeast research Pub Date : 2025-01-30 DOI:10.1093/femsyr/foaf013

Nicholas R Robertson, Sangcheon Lee, Aida Tafrishi, Ian Wheeldon

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引用次数: 0

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas genome-wide screens are powerful tools for unraveling genotype-phenotype relationships, enabling precise manipulation of genes to study and engineer industrially useful traits. Traditional genetic methods, such as random mutagenesis or RNA interference, often lack the specificity and scalability required for large-scale functional genomic screens. CRISPR systems overcome these limitations by offering precision gene targeting and manipulation, allowing for high-throughput investigations into gene function and interactions. Recent work has shown that CRISPR genome editing is widely adaptable to several yeast species, many of which have natural traits suited for industrial biotechnology. In this review, we discuss recent advances in yeast functional genomics, emphasizing advancements made with CRISPR tools. We discuss how the development and optimization of CRISPR genome-wide screens have enabled a host-first approach to metabolic engineering, which takes advantage of the natural traits of nonconventional yeast-fast growth rates, high stress tolerance, and novel metabolism-to create new production hosts. Lastly, we discuss future directions, including automation and biosensor-driven screens, to enhance high-throughput CRISPR-enabled yeast engineering.

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酵母中 CRISPR 驱动的全基因组筛选的进展。

CRISPR-Cas全基因组筛选是揭示基因型-表型关系的强大工具，可以精确操纵基因来研究和设计工业上有用的性状。传统的遗传方法，如随机诱变或RNA干扰，往往缺乏大规模功能基因组筛选所需的特异性和可扩展性。CRISPR系统通过提供精确的基因靶向和操作来克服这些限制，允许对基因功能和相互作用进行高通量研究。最近的研究表明，CRISPR基因组编辑广泛适用于几种酵母菌，其中许多酵母菌具有适合工业生物技术的自然特性。在这篇综述中，我们讨论了酵母功能基因组学的最新进展，重点介绍了CRISPR工具的进展。我们讨论了CRISPR全基因组筛选的开发和优化如何实现宿主优先的代谢工程方法，该方法利用非常规酵母的自然特性-快速生长速率，高耐受性和新型代谢-来创造新的生产宿主。最后，我们讨论了未来的方向，包括自动化和生物传感器驱动的屏幕，以增强高通量crispr酵母工程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

FEMS yeast research 生物-生物工程与应用微生物

CiteScore

5.70

自引率

6.20%

发文量

审稿时长

1 months

期刊介绍： FEMS Yeast Research offers efficient publication of high-quality original Research Articles, Mini-reviews, Letters to the Editor, Perspectives and Commentaries that express current opinions. The journal will select for publication only those manuscripts deemed to be of major relevance to the field and generally will not consider articles that are largely descriptive without insights on underlying mechanism or biology. Submissions on any yeast species are welcome provided they report results within the scope outlined below and are of significance to the yeast field.