Amniotic fluid stem cell conditioned medium's role in Schwann cell proliferation, survival, and cellular antioxidant activity under normative and oxidative stress conditions.

4区 医学
Annals of translational medicine Pub Date : 2025-02-28 Epub Date: 2025-02-25 DOI:10.21037/atm-24-107
Chukwuweike Gwam, Nequesha Mohamed, Ayobami S Ogunsola, Marcel G Brown, Kaitlin A Henry, Xue Ma
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引用次数: 0

Abstract

Background: Peripheral nerve injuries present a major clinical challenge due to their high morbidity and often incomplete recovery of function. While autografts remain the gold standard for nerve repair, their use is constrained by limited donor availability and donor site complications. Alternative strategies, such as allografts and tissue-engineered grafts, have been developed but are still associated with suboptimal outcomes, including chronic pain and sensory disturbances. Thus, there is a need for novel therapies that can enhance nerve regeneration. Amniotic fluid stem cell conditioned medium (AFS-CM) houses regenerative properties that may be useful in peripheral nerve injury. This study aims to assess the role of AFS-CM on Schwann cell survival and proliferation under normative and oxidative stress conditions, preventing oxidative stress-induced premature senescence of Schwann cells in vitro and maintaining cellular redox homeostasis.

Methods: Primary Schwann cells were treated with various concentrations of AFS-CM. Cell proliferation was assessed using the Cell Counting Kit-8 (CCK-8) assay, and viability under oxidative stress was measured after exposing cells to hydrogen peroxide (H2O2). Reactive oxygen species (ROS) levels and both catalase and superoxide dismutase (SOD) levels were evaluated. Cellular senescence markers were also assessed to determine AFS-CM's protective effects.

Results: AFS-CM treatment resulted in a dose-dependent increase in Schwann cell proliferation (P<0.05). Under oxidative stress conditions, AFS-CM significantly improved cell viability compared to controls (P<0.05). ROS levels were markedly reduced in AFS-CM-treated cells (P<0.05), and this was accompanied by upregulation of catalase and SOD expression (P<0.05). Moreover, AFS-CM reduced stress-induced cellular senescence, as indicated by decreased senescence-associated β-galactosidase activity and lower expression of senescence markers (P<0.05).

Conclusions: AFS-CM enhances Schwann cell proliferation, viability, and resistance to oxidative stress, while reducing cellular senescence. These findings suggest that AFS-CM could be a promising adjunctive therapy for peripheral nerve injuries by promoting Schwann cell resilience and regenerative capacity. Future studies are needed to validate these in vitro results in vivo and explore their potential clinical application for improving functional recovery in patients with peripheral nerve damage.

背景:周围神经损伤是一项重大的临床挑战,因为其发病率高且功能往往无法完全恢复。虽然自体移植物仍是神经修复的黄金标准,但其使用受到供体有限和供体部位并发症的限制。异体移植物和组织工程移植物等替代方法已被开发出来,但其效果仍不理想,包括慢性疼痛和感觉障碍。因此,我们需要能促进神经再生的新型疗法。羊水干细胞条件培养基(AFS-CM)具有再生特性,可用于周围神经损伤。本研究旨在评估 AFS-CM 在正常和氧化应激条件下对许旺细胞存活和增殖的作用,防止氧化应激诱导的体外许旺细胞过早衰老,维持细胞氧化还原平衡:方法:用不同浓度的 AFS-CM 处理原代许旺细胞。方法:用不同浓度的 AFS-CM 处理原发性许旺细胞,使用细胞计数试剂盒-8(CCK-8)测定法评估细胞增殖情况,并在细胞暴露于过氧化氢(H2O2)后测定细胞在氧化应激下的存活率。评估了活性氧(ROS)水平以及过氧化氢酶和超氧化物歧化酶(SOD)水平。还评估了细胞衰老标记物,以确定 AFS-CM 的保护作用:结果:AFS-CM 处理导致许旺细胞增殖(PConclusions:AFS-CM能增强许旺细胞的增殖、活力和对氧化应激的抵抗力,同时减少细胞衰老。这些研究结果表明,AFS-CM 可以促进许旺细胞的恢复能力和再生能力,是一种很有前景的外周神经损伤辅助疗法。未来的研究需要在体内验证这些体外结果,并探索它们在改善周围神经损伤患者功能恢复方面的潜在临床应用。
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来源期刊
自引率
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发文量
769
期刊介绍: The Annals of Translational Medicine (Ann Transl Med; ATM; Print ISSN 2305-5839; Online ISSN 2305-5847) is an international, peer-reviewed Open Access journal featuring original and observational investigations in the broad fields of laboratory, clinical, and public health research, aiming to provide practical up-to-date information in significant research from all subspecialties of medicine and to broaden the readers’ vision and horizon from bench to bed and bed to bench. It is published quarterly (April 2013- Dec. 2013), monthly (Jan. 2014 - Feb. 2015), biweekly (March 2015-) and openly distributed worldwide. Annals of Translational Medicine is indexed in PubMed in Sept 2014 and in SCIE in 2018. Specific areas of interest include, but not limited to, multimodality therapy, epidemiology, biomarkers, imaging, biology, pathology, and technical advances related to medicine. Submissions describing preclinical research with potential for application to human disease, and studies describing research obtained from preliminary human experimentation with potential to further the understanding of biological mechanism underlying disease are encouraged. Also warmly welcome are studies describing public health research pertinent to clinic, disease diagnosis and prevention, or healthcare policy.
 With a focus on interdisciplinary academic cooperation, ATM aims to expedite the translation of scientific discovery into new or improved standards of management and health outcomes practice.
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