Identification of Potential Targets Associated With Programmed Cell Death for Acute Kidney Injury Based on WGCNA.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Yu Wang, Bo Deng, Yu Pan, Feng Ding
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引用次数: 0

Abstract

Programmed cell death (PCD) pathways play a crucial role in maintaining normal cell turnover and tissue homeostasis, encompassing apoptosis and regulated necrosis. However, the involvement of PCD in the pathogenesis of acute kidney disease remains unexplored. In this study, we utilized gene expression profiling datasets (GSE139061) obtained from the Gene Expression Omnibus (GEO) database. Through differential gene expression analysis and weighted gene co-expression network analysis (WGCNA), we identified five key genes associated with PCD, namely DPP4, ATF3, KIT, MSX1, and SNAI2 in acute kidney injury (AKI). Subsequently, single sample gene set enrichment analysis (ssGSEA) was employed to demonstrate the correlation between these five hub genes and immune cell infiltration as well as activation of immune pathways. Furthermore, we validated our findings by analyzing gene expression patterns using a mouse model of ischemia-reperfusion injury. In conclusion, our study is the first to propose the concept of PCD in the pathogenesis of AKI. This finding has significant implications for future investigations into pro-inflammatory immune mechanisms mediated by damage-associated molecular patterns (DAMPs) during the stages of AKI. Our findings underscore the necessity for further investigation into these molecules, which may offer new avenues for therapeutic intervention in AKI. These identified genes may serve as promising targets for intervention in cases of acute kidney diseases.

基于WGCNA的急性肾损伤程序性细胞死亡相关潜在靶点鉴定
程序性细胞死亡(PCD)途径在维持正常细胞更新和组织稳态中起着至关重要的作用,包括细胞凋亡和受调节的坏死。然而,PCD在急性肾脏疾病发病机制中的作用仍未被探索。在本研究中,我们使用了从gene expression Omnibus (GEO)数据库中获得的基因表达谱数据集(GSE139061)。通过差异基因表达分析和加权基因共表达网络分析(WGCNA),我们确定了急性肾损伤(AKI)中与PCD相关的5个关键基因,分别是DPP4、ATF3、KIT、MSX1和SNAI2。随后,采用单样本基因集富集分析(ssGSEA)证实了这五个中心基因与免疫细胞浸润和免疫通路激活的相关性。此外,我们通过使用小鼠缺血再灌注损伤模型分析基因表达模式来验证我们的发现。总之,我们的研究首次提出了PCD在AKI发病机制中的概念。这一发现对未来AKI阶段损伤相关分子模式(DAMPs)介导的促炎免疫机制的研究具有重要意义。我们的发现强调了进一步研究这些分子的必要性,这可能为AKI的治疗干预提供新的途径。这些已鉴定的基因可能成为急性肾脏疾病病例干预的有希望的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell Biology International
Cell Biology International 生物-细胞生物学
CiteScore
7.60
自引率
0.00%
发文量
208
审稿时长
1 months
期刊介绍: Each month, the journal publishes easy-to-assimilate, up-to-the minute reports of experimental findings by researchers using a wide range of the latest techniques. Promoting the aims of cell biologists worldwide, papers reporting on structure and function - especially where they relate to the physiology of the whole cell - are strongly encouraged. Molecular biology is welcome, as long as articles report findings that are seen in the wider context of cell biology. In covering all areas of the cell, the journal is both appealing and accessible to a broad audience. Authors whose papers do not appeal to cell biologists in general because their topic is too specialized (e.g. infectious microbes, protozoology) are recommended to send them to more relevant journals. Papers reporting whole animal studies or work more suited to a medical journal, e.g. histopathological studies or clinical immunology, are unlikely to be accepted, unless they are fully focused on some important cellular aspect. These last remarks extend particularly to papers on cancer. Unless firmly based on some deeper cellular or molecular biological principle, papers that are highly specialized in this field, with limited appeal to cell biologists at large, should be directed towards journals devoted to cancer, there being very many from which to choose.
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