Three polysaccharides from Roughhaired Holly Root with immune-enhancing activity via activation of TLR receptor

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-05-01 DOI:10.1016/j.ijbiomac.2025.142266

Rong Huang , Xiaoli Huang , Weining He , Zhangfeng Zhong , Haiqin Li , Qiu Li

{"title":"Three polysaccharides from Roughhaired Holly Root with immune-enhancing activity via activation of TLR receptor","authors":"Rong Huang , Xiaoli Huang , Weining He , Zhangfeng Zhong , Haiqin Li , Qiu Li","doi":"10.1016/j.ijbiomac.2025.142266","DOIUrl":null,"url":null,"abstract":"<div><div>In this study, we isolated three acidic polysaccharide components (IAPS-1, IAPS-2, and IAPS-3) from Rough-haired Holly (<em>Ilex asprella</em>) roots and systematically evaluated their immunomodulatory activity in murine macrophages. The polysaccharides were structurally characterized using integrated chromatographic and spectroscopic techniques. Subsequent bioactivity screening revealed that IAPS-1 demonstrated stronger immunomodulatory effects compared to IAPS-2 and IAPS-3. Mechanistically, in vitro experiments showed that IAPS-1 significantly upregulated TLR4-mediated expression of pro-inflammatory cytokines in RAW 264.7 macrophages. Furthermore, in cyclophosphamide-induced immunosuppressed mice, IAPS-1 administration restored immune organ indices and elevated serum levels of critical inflammatory mediators. Collectively, our findings identify IAPS-1 as a well-defined composition polysaccharide with immunomodulatory activity via TLR4 signaling, highlighting its potential as a therapeutic candidate for immune dysfunction.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"308 ","pages":"Article 142266"},"PeriodicalIF":7.7000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biological Macromolecules","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0141813025028181","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

In this study, we isolated three acidic polysaccharide components (IAPS-1, IAPS-2, and IAPS-3) from Rough-haired Holly (Ilex asprella) roots and systematically evaluated their immunomodulatory activity in murine macrophages. The polysaccharides were structurally characterized using integrated chromatographic and spectroscopic techniques. Subsequent bioactivity screening revealed that IAPS-1 demonstrated stronger immunomodulatory effects compared to IAPS-2 and IAPS-3. Mechanistically, in vitro experiments showed that IAPS-1 significantly upregulated TLR4-mediated expression of pro-inflammatory cytokines in RAW 264.7 macrophages. Furthermore, in cyclophosphamide-induced immunosuppressed mice, IAPS-1 administration restored immune organ indices and elevated serum levels of critical inflammatory mediators. Collectively, our findings identify IAPS-1 as a well-defined composition polysaccharide with immunomodulatory activity via TLR4 signaling, highlighting its potential as a therapeutic candidate for immune dysfunction.

查看原文本刊更多论文

粗毛冬青根中的三种多糖通过激活 TLR 受体具有免疫增强活性。

本研究从毛毛冬青（Ilex asprella）根中分离出三种酸性多糖成分IAPS-1、IAPS-2和IAPS-3，并系统评价了它们对小鼠巨噬细胞的免疫调节活性。采用色谱和光谱综合技术对多糖进行了结构表征。随后的生物活性筛选显示IAPS-1比IAPS-2和IAPS-3具有更强的免疫调节作用。在机制上，体外实验表明IAPS-1显著上调tlr4介导的RAW 264.7巨噬细胞中促炎细胞因子的表达。此外，在环磷酰胺诱导的免疫抑制小鼠中，IAPS-1可恢复免疫器官指数和升高血清中关键炎症介质的水平。总的来说，我们的研究结果确定IAPS-1是一种明确的组合多糖，通过TLR4信号具有免疫调节活性，突出了其作为免疫功能障碍治疗候选药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.