Structural characterization and combined immunomodulatory activity of fermented Chinese yam polysaccharides with probiotics.

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-05-01 Epub Date: 2025-03-19 DOI:10.1016/j.ijbiomac.2025.142290

Yahui Yang, Ziru Lai, Xilei Hu, Peiyao Zhang, Yang Zhang, Yi Zheng, Li Ding, Jiaqi Wang, Na Li, Zichao Wang, Lemei An, Yi Ding

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Abstract

In this study, Lactobacillus plantarum M616 and Saccharomyces cerevisiae CICC 32883 were used in modifying Chinese yam polysaccharides (CYPs) through fermentation. The carbohydrate content of microbe-fermented CYP (CYP-LS) was 78.49 % ± 1.64 %, versus the 71.03 % ± 2.75 % carbon content of unfermented CYP (CYP-NF). However, CYP-LS had a lower protein content (6.01 % ± 0.08 %) than CYP-NF (8.24 % ± 0.19 %). The molar ratios among rhamnose, arabinose, galactose, glucose, and mannose were respectively 0.493:0.6695:0.9738:0.7655:12.4365 for CYP-NF and 0.2849:0.182:0.5684:1.4069:3.7227 for CYP-LS. Molecular weight and polydispersity decreased respectively from 124.774 kDa (CYP-NF) to 34.111 kDa (CYP-LS) to and from 6.58 (CYP-NF) to 5.176 (CYP-LS). Moreover, CYP-LS had better immunomodulatory activity than CYP-NF, regulating superoxide dismutase, catalase, glutathione peroxidase, malondialdehyde, tumor necrosis factor-α, interleukin-1β, interleukin-10, and transforming growth factor-β in a RAW 264.7 cell model. A combination of CYP-LS and probiotics (Lactobacillus helveticus HH-LPH17, Lactobacillus johnsonii LBJ 456® and Lactobacillus acidophilus HH-LA26) showed enhanced immune activity.

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发酵山药多糖与益生菌的结构特征及联合免疫调节活性

本研究利用植物乳杆菌 M616 和酿酒酵母 CICC 32883 通过发酵改良山药多糖（CYP）。经微生物发酵的山药多糖（CYP-LS）的碳水化合物含量为 78.49 % ± 1.64 %，而未经发酵的山药多糖（CYP-NF）的碳含量为 71.03 % ± 2.75 %。不过，CYP-LS 的蛋白质含量（6.01 % ± 0.08 %）低于 CYP-NF（8.24 % ± 0.19 %）。鼠李糖、阿拉伯糖、半乳糖、葡萄糖和甘露糖的摩尔比分别为：CYP-NF 0.493:0.6695:0.9738:0.7655:12.4365，CYP-LS 0.2849:0.182:0.5684:1.4069:3.7227。分子量和多分散性分别从 124.774 kDa（CYP-NF）降至 34.111 kDa（CYP-LS）和从 6.58（CYP-NF）降至 5.176（CYP-LS）。此外，在 RAW 264.7 细胞模型中，CYP-LS 比 CYP-NF 具有更好的免疫调节活性，能调节超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、丙二醛、肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-10 和转化生长因子-β。CYP-LS 与益生菌（螺旋乳杆菌 HH-LPH17、约翰逊乳杆菌 LBJ 456® 和嗜酸乳杆菌 HH-LA26）的组合显示出更强的免疫活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.