Biological Production and Structural Characterization of PHB from Commercial Sugarcane Molasses

IF 4.7 3区工程技术 Q2 ENGINEERING, ENVIRONMENTAL

Journal of Polymers and the Environment Pub Date : 2025-01-31 DOI:10.1007/s10924-025-03519-2

Débora J. N. de Melo, Lucineide B. da Silva, Sharline F. M. Santos

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Abstract

World population growth associated with unbridled consumption have led to notable increases in the use of petroleum-based plastics. Biodegradable polymers produced from sustainable and low-cost raw materials have been emerging as a key solution. The objective of this work was to obtain a polymer of microbial origin from an alternative and renewable source. Bacterial Polyhydroxybutyrate (PHB-B) was produced by submerged culture of Cupriavidus necator using commercial sugarcane molasses (CSM) as a renewable substrate. A 2² factorial experimental design with three central point replications was conducted to identify the most favorable culture conditions. The 15 g.L⁻¹ concentration of reducing sugar (RS) obtained from the CSM was effective as a carbon source, yielding (under stirring at 180 rpm and 30 °C) a maximum production of 35.70% PHB-B, while presenting the same functional groups and crystal structure as commercial grade Polyhydroxybutyrate (PHB-C). The intensities obtained for the 2933 and 2975 cm⁻¹ bands, suggested that PHB-C presents higher crystallinity than PHB-B. ¹H and ¹³C NMR confirmed the identity of the bacterial biopolymer as Polyhydroxybutyrate. The diffraction peaks were less intense for PHB-B, and the peaks at (021) and (101) coalesced into a single peak at (111). The peak shapes indicated that PHB-B presents smaller and/or less perfect crystals than PHB-C. Considering the observed structural differences, the rigidity and flexibility of the biopolymers may differ. Bacterial PHB was produced using a low-pollution route. This resulted in a product which potentially expands the range of environmentally friendly PHB applications.

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商用甘蔗糖蜜 PHB 的生物生产和结构表征

世界人口增长与无节制的消费相关联，导致石油基塑料的使用显著增加。由可持续和低成本的原材料生产的可生物降解聚合物已经成为一个关键的解决方案。这项工作的目的是从可替代和可再生的来源获得微生物来源的聚合物。以商业甘蔗糖蜜（CSM）为可再生底物，对Cupriavidus necator进行深层培养制备细菌聚羟基丁酸盐（PHB-B）。采用22因子试验设计，三个中心点重复，以确定最有利的培养条件。15g。从CSM中得到的L−1浓度的还原糖（RS）作为碳源是有效的，在180转/分和30°C搅拌下，PHB-B的最大产量为35.70%，而其官能团和晶体结构与商业级聚羟基丁酸酯（PHB-C）相同。2933和2975 cm−1波段的强度表明，PHB-C的结晶度高于PHB-B。1H和13C核磁共振证实该细菌生物聚合物为聚羟基丁酸酯。PHB-B的衍射峰强度较小，在（021）和（101）处的峰在（111）处合并为一个单峰。峰形表明PHB-B的晶体比PHB-C更小或更不完美。考虑到观察到的结构差异，生物聚合物的刚性和柔韧性可能不同。采用低污染途径生产细菌PHB。这就产生了一种可能扩大环境友好型PHB应用范围的产品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Polymers and the Environment 工程技术-高分子科学

CiteScore

9.50

自引率

7.50%

发文量

297

审稿时长

9 months

期刊介绍： The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.