Machine Learning-Assisted Portable Dual-Readout Biosensor for Visual Detection of Milk Allergen

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-03-20 DOI:10.1021/acs.nanolett.5c01001

Jing Xu, Xinqi Luo, Mohan Duan, Kexin Guo, Yuxiao Shangguan, Qingle Zhao, Minyu Qiu, Fu Wang

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Abstract

Beta-lactoglobulin (β-LG), the primary allergen in cow’s milk, makes developing a rapid, sensitive, and convenient detection method essential for individuals with allergies. In this study, a graphdiyne-based self-powered electrochemical biosensor has been cleverly integrated into the corresponding test strip. This biosensor uses glucose as fuel and correlates the β-LG concentration with the glucose value displayed on a mobile phone application, enabling real-time and quantitative detection. Additionally, an electrochromic substance reacts with the byproduct (H₂O₂) of glucose oxidation by biological enzymes. A quantitative relationship between color and β-LG concentration has been established using mobile phone software. Dual detection of electrochemical and colorimetric signals in the 0.01–10,000 ng/mL range, with detection limits as low as 0.0033 and 0.0081 ng/mL, is possible. Machine learning is finally employed to analyze its performance. Our dual-readout biosensor demonstrates significant potential for rapid food allergy detection.

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用于牛奶过敏原视觉检测的机器学习辅助便携式双读数生物传感器

β-乳球蛋白（β-LG）是牛奶中的主要过敏原，因此开发一种快速、灵敏、方便的检测方法对过敏个体至关重要。在这项研究中，基于石墨烯的自供电电化学生物传感器被巧妙地集成到相应的测试条中。这种生物传感器以葡萄糖为燃料，并将β-LG浓度与显示在手机应用程序上的葡萄糖值相关联，从而实现实时定量检测。此外，电致变色物质与生物酶氧化葡萄糖的副产物（H2O2）发生反应。利用手机软件建立了颜色与β-LG浓度之间的定量关系。在0.01 ~ 10,000 ng/mL范围内可实现电化学和比色信号的双重检测，检出限低至0.0033和0.0081 ng/mL。最后利用机器学习对其性能进行分析。我们的双读数生物传感器显示出快速检测食物过敏的巨大潜力。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.