Chrysin ameliorates pain through regulation of the serum metabolomics in the rats.

IF 3.4 3区医学 Q2 CLINICAL NEUROLOGY

Korean Journal of Pain Pub Date : 2025-04-01 Epub Date: 2025-03-20 DOI:10.3344/kjp.24355

Khadijeh Haghighat, Fariba Mahmoudi, Maryam Khoshkam, Homayoun Khazali

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引用次数: 0

Abstract

Background: Chrysin is a natural flavonoid that exhibits various pharmacological activities including pain relief. However, the effects of chrysin on changes of metabolic profiles during pain remain unclear. The aim of this study was to analyze the biomarkers related to pain in serum and to evaluate the analgesic properties of chrysin in a rat model of pain.

Methods: Male Wister rats were divided into four groups (n = 5). Pain was induced by injecting 50 μL of formalin into the hind paw. Chrysin and diclofenac (10 mg/kg, intraperitoneal injection) was administered to the intact and pain groups. All injections were given 30 minutes before pain induction. Immediately, the behavioral test was performed. Then the serum sample was separated for ¹HNMR-based metabolite analysis.

Results: Chrysin treatment alleviated the paw licking events, flinching response, and pain score. The integrated analyses further revealed three major metabolic changes including glycine-serine-threonine, taurine-hypotaurine, and arginine by comparing the serums from intact operated rats, pain rats, and pain rats treated with chrysin, and suggested that chrysin may improve pain by regulating the biosynthesis of these metabolic pathways.

Conclusions: These findings provide insights into metabolic pathways involved in pain and the analgesic effects of chrysin and may help to identify potential targets for the anti-pain properties of chrysin.

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黄菊花素通过调节大鼠血清代谢组学改善疼痛。

背景：菊花素是一种天然类黄酮，具有多种药理活性，包括镇痛。然而，黄菊花素对疼痛期间代谢谱变化的影响尚不清楚。本研究的目的是分析血清中与疼痛相关的生物标志物，并评估菊花素在疼痛大鼠模型中的镇痛特性。方法：雄性Wister大鼠随机分为4组（n = 5），后爪注射50 μL福尔马林致痛。正常组和疼痛组分别给予巨霉素和双氯芬酸（10 mg/kg，腹腔注射）。所有注射均在疼痛诱导前30分钟进行。立即进行了行为测试。然后分离血清样本进行基于1hnmr的代谢物分析。结果：金菊素治疗可减轻舔爪事件、退缩反应和疼痛评分。通过对手术大鼠、疼痛大鼠和金菊素治疗的疼痛大鼠的血清进行综合分析，进一步揭示了甘氨酸-丝氨酸-苏氨酸、牛磺酸-次牛磺酸和精氨酸三种主要代谢变化，并提示金菊素可能通过调节这些代谢途径的生物合成来改善疼痛。结论：这些发现有助于深入了解菊花素镇痛作用和疼痛的代谢途径，并有助于确定菊花素镇痛作用的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Korean Journal of Pain Medicine-Anesthesiology and Pain Medicine

CiteScore

5.40

自引率

7.10%

发文量

审稿时长

16 weeks

期刊介绍： Korean Journal of Pain (Korean J Pain, KJP) is the official journal of the Korean Pain Society, founded in 1986. It has been published since 1988. It publishes peer reviewed original articles related to all aspects of pain, including clinical and basic research, patient care, education, and health policy. It has been published quarterly in English since 2009 (on the first day of January, April, July, and October). In addition, it has also become the official journal of the International Spinal Pain Society since 2016. The mission of the Journal is to improve the care of patients in pain by providing a forum for clinical researchers, basic scientists, clinicians, and other health professionals. The circulation number per issue is 50.