Protective effect of polyphenols from Potentilla anserina L. against in rat myocardial ischemia-reperfusion on injury by Wnt/β-catenin pathway.

Abstract

Myocardial ischemia reperfusion injury (MIRI) remains a major clinical challenge. Polyphenols derived from Potentilla anserina L (PPA) were found to possess anti-hypoxic-ischemic in vitro. The aim of this study was to examine (1) whether this plant exerts a protective effect and (2) whether the underlying mechanisms involving inflammatory responses and the Wnt/β-catenin signaling pathway were involved in rats. Rats were randomly divided as follows (1): Sham operation (2); ischemia-reperfusion (MIRI) (3); polyphenols (PPA) (4); Wnt inhibitor group (XAV939). In cardiomyocytes (1), HE staining was used to examine morphology (2), enzyme-linked immunosorbent assay to determine interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels and (3) Western blot to measure protein expression of Wnt and β-catenin. HE staining showed in MIRI edema of cardiomyocytes, localized inflammatory cell infiltration, unclear outline of cells and cell fragmentation, and some cardiomyocytes were seen to have disordered arrangement of tissues. PPA markedly lowered the morphological alterations in MIRI. The levels of levels of IL-1β and TNF-α in MIRI were significantly elevated; however, PPA reduced these cytokine concentrations compared to MIRI. Western blot analysis demonstrated that MIRI increased the relative protein expression levels of Wnt and β-catenin. Treatment of MIRI with PPA resulted in a significant decrease in protein expression levels of Wnt and β-catenin. The involvement of the Wnt/β-catenin pathway in MIRI was further affirmed with the use Wnt inhibitor group (XAV939) that also diminished protein expression levels of Wnt and β-catenin.