Swertianolin regulates immunosuppression of myeloid suppressor cells in septic mice by inhibiting NF-κB and P38 signaling

IF 1.6 4区医学 Q4 IMMUNOLOGY

Transplant immunology Pub Date : 2025-03-17 DOI:10.1016/j.trim.2025.102217

Haoran Tang , Chen Liao , Lingling Wang , Wei Fang , Ning Tang , Linjun Wan , Zongfang Ren

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引用次数: 0

Abstract

Background

Swertianolin is one of the main components of Gentianaceae Swertia plants, a traditional Chinese medicine used for the treatment of infection, fever, viral hepatitis, and pneumonia. An expansion of myeloid-derived suppressor cells (MDSCs) contributes to sepsis induced immunosuppression. We investigated the mechanism by which Swertianolin regulates MDSCs in a mouse model of sepsis.

Methods

Severe sepsis was induced in mice using caecal ligation and puncture. These mice received an intraperitoneal injection of Swertianolin. MDSCs were isolated and analyzed by flow cytometry; serum concentrations of immunosuppressive factors were detected by ELISA; and mitogen-activated protein kinase and nuclear factor-κB (NFκB) were detected by Western blots.

Results

We found that Swertianolin reduced the number of MDSCs in the marrow and the spleen while increased the number of CD4+ T cells in the spleen of mice with sepsis in comparison to controls (p < 0.05). Swertianolin reduced lung damage and improved the survival rate in mice with secondary infection of Legionella pneumophila (p < 0.05). Swertianolin inhibited the phosphorylation of p38 and nuclear translocation of p65 in MDSCs (p < 0.05), leading to decreased production of IL-10 and nitric oxide (both p < 0.05).

Conclusion

Swertianolin may improve immunosuppressive function of MDSCs and increased T cell activity by inhibiting p38 phosphorylation and NF-κB activation.

查看原文本刊更多论文

獐牙菜苷通过抑制NF-κB和P38信号传导调节脓毒症小鼠骨髓抑制细胞的免疫抑制。

背景：獐牙菜苷是龙胆科獐牙菜属植物的主要成分之一，是一种用于治疗感染、发热、病毒性肝炎和肺炎的中药。骨髓源性抑制细胞（MDSCs）的扩增有助于脓毒症诱导的免疫抑制。我们在脓毒症小鼠模型中研究了獐牙菜苷调节MDSCs的机制。方法：采用结扎法和穿刺法诱导小鼠严重脓毒症。这些小鼠接受了獐牙菜苷腹腔注射。分离MDSCs，流式细胞术分析；ELISA法检测血清免疫抑制因子浓度；Western blot检测丝裂原活化蛋白激酶和核因子-κB （NFκB）的表达。结果：我们发现，与对照组相比，Swertianolin减少了脓毒症小鼠骨髓和脾脏中MDSCs的数量，同时增加了脾脏中CD4+ T细胞的数量（p ）。结论：Swertianolin可能通过抑制p38磷酸化和NF-κB活化来改善MDSCs的免疫抑制功能，增加T细胞的活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transplant immunology 医学-免疫学

CiteScore

2.10

自引率

13.30%

发文量

198

审稿时长

48 days

期刊介绍： Transplant Immunology will publish up-to-date information on all aspects of the broad field it encompasses. The journal will be directed at (basic) scientists, tissue typers, transplant physicians and surgeons, and research and data on all immunological aspects of organ-, tissue- and (haematopoietic) stem cell transplantation are of potential interest to the readers of Transplant Immunology. Original papers, Review articles and Hypotheses will be considered for publication and submitted manuscripts will be rapidly peer-reviewed and published. They will be judged on the basis of scientific merit, originality, timeliness and quality.