Microbiome Signatures and Dysbiotic Patterns in Oral Cancer and Precancerous Lesions.

IF 2.9 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Oral diseases Pub Date : 2025-03-19 DOI:10.1111/odi.15317
Cheng-Chieh Yang, Jumpei Washio, Yu-Cheng Lin, Ming-Lun Hsu, Ding-Han Wang, Fa-Tzu Tsai, Yu-Min Lin, Hsi-Feng Tu, Hsiu-Chuan Chang, Nobuhiro Takahashi
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引用次数: 0

Abstract

Background: The oral microbiome has been shown to be associated with the development of oral squamous cell carcinoma (OSCC). Research has primarily focused on elucidating the oncogenic mechanisms of specific pathogens by comparing the microbiomes of OSCC and normal tissues. However, the characteristics of the microbiome in the precancerous state remain less understood, as does the influence of metabolic and environmental factors on OSCC-associated microbiomes.

Methods: In this study, we analyzed mucosa-associated microbiomes in normal, precancerous, and OSCC lesions from a cohort of 51 patients using 16S rRNA amplicon sequencing. We investigated compositional changes in the microbiome, including the specific abundances and co-occurrences of OSCC-associated bacteria.

Results: Our findings indicate that the microbiome associated with precancerous lesions is indistinguishable from that of the normal mucosa, whereas the OSCC microbiome significantly differs from both normal and precancerous conditions. Specifically, the OSCC microbiome harbors less Streptococcus, coupled with an increase in amino-acid-degrading anaerobes such as Fusobacterium and Prevotella. The metabolic properties of individual microbes reported suggest that the overrepresentation of OSCC-specific bacteria is a result of metabolic adaptation to tumor microenvironments, although this possibility needs to be experimentally confirmed.

Conclusions: Our results demonstrate oral microbiome patterns across OSCC progression, offering insights into microbial ecological perspectives.

口腔癌和癌前病变的微生物组特征和生态失调模式。
背景:口腔微生物组已被证明与口腔鳞状细胞癌(OSCC)的发展有关。研究主要集中在通过比较OSCC和正常组织的微生物组来阐明特定病原体的致癌机制。然而,癌前状态下微生物组的特征仍然知之甚少,代谢和环境因素对oscc相关微生物组的影响也是如此。方法:在这项研究中,我们使用16S rRNA扩增子测序分析了51例正常、癌前病变和OSCC病变中粘膜相关微生物组。我们研究了微生物组的组成变化,包括oscc相关细菌的特定丰度和共现。结果:我们的研究结果表明,与癌前病变相关的微生物组与正常粘膜的微生物组难以区分,而OSCC的微生物组与正常和癌前病变都有显著差异。具体来说,OSCC微生物群中含有较少的链球菌,同时含有较多的氨基酸降解厌氧菌,如梭杆菌和普雷沃氏菌。报道的个体微生物的代谢特性表明,oscc特异性细菌的过度代表是对肿瘤微环境的代谢适应的结果,尽管这种可能性需要实验证实。结论:我们的研究结果展示了口腔微生物组在OSCC进展过程中的模式,为微生物生态学的观点提供了见解。
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来源期刊
Oral diseases
Oral diseases 医学-牙科与口腔外科
CiteScore
7.60
自引率
5.30%
发文量
325
审稿时长
4-8 weeks
期刊介绍: Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.
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