A comparative analysis of the impact of repeated administration of flavan 3-ol on brown, subcutaneous, and visceral adipose tissue.

IF 1.7 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Open Medicine Pub Date : 2025-03-18 eCollection Date: 2025-01-01 DOI:10.1515/med-2025-1152

Naomi Osakabe, Hitomi Nakamura, Yamato Yoshida, Sae Katsuragawa, Naoki Iida, Yasuyuki Fujii, Ursula M Jacob, Tilman Fritsch, Ali Abdelhameed, Vittorio Calabrese

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引用次数: 0

Abstract

Introduction: Flavan-3-ols (FLs), astringent polyphenols, are known to have low bioavailability and induce excessive sympathetic nervous system activation. This study aimed to compare the effects of FLs on brown, subcutaneous, and visceral adipose tissue in mice.

Methods: C57BL/6J male mice fed a standard or high-fat diet were given water or 50 mg/kg FL orally by gavage for 2 weeks. Excised brown, inguinal, and epididymal fat tissues were prepared for frozen sectioning. After hematoxylin and eosin (HE) staining, the effects of FL administration on each adipose tissue were observed, and expression analysis of mitochondrial DNA genes was performed.

Results: Repeated administration of FL had no morphological effects on brown adipose tissue or visceral fat. However, FL significantly reduced the cell size in subcutaneous fat and induced the appearance of multilocular structures. Furthermore, FL increased cytochrome B expression in subcutaneous fat. The results showed that FLs induce browning of subcutaneous fat in mice.

Conclusion: This study showed that FL-induced enhancement of sympathetic nerve activity increased mitochondria in subcutaneous fat and promoted browning. However, no changes were observed in other adipose tissues. Further long-term administration is required to analyze the effects of FLs on adipose tissue thoroughly.

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来源期刊

Open Medicine Medicine-General Medicine

CiteScore

3.00

自引率

0.00%

发文量

153

审稿时长

20 weeks

期刊介绍： Open Medicine is an open access journal that provides users with free, instant, and continued access to all content worldwide. The primary goal of the journal has always been a focus on maintaining the high quality of its published content. Its mission is to facilitate the exchange of ideas between medical science researchers from different countries. Papers connected to all fields of medicine and public health are welcomed. Open Medicine accepts submissions of research articles, reviews, case reports, letters to editor and book reviews.