Two new bergamotane-type sesquiterpeniods from the plant endophytic fungus Paraconiothyrium brasiliense.

Abstract

One new bergamotane-type sesquiterpeniod lactam brasilactam A (1) and one new bergamotane-type sesquiterpeniod spiroketal lactone brasiketalolide A (2), along with two known compounds ampullicin (3) and isoampullicin (4) were isolated from the crude extract of the plant endophytic fungus Paraconiothynium brasiliense Verkley. The structures of the new compounds were elucidated on the basis of extensive 2D NMR techniques. The absolute configurations of 1 and 2 were assigned by ¹³C NMR and electronic circular dichroism (ECD) calculations. Furthermore, compounds 1 - 4 were tested for their cytotoxicity against four human cancer cell lines.