Knowledge, attitudes, and perception of dog owners on the transmission, control and prevention of cystic echinococcosis and other gastrointestinal parasites in dogs of Southern provinces of Mozambique.

Abstract

In rural communities of Mozambique, dogs are utilized not only as companion animals, but also for shepherding livestock, guarding, and hunting purposes, increasing exposure of humans to zoonotic parasites. Thus, we conducted a study to assess knowledge, attitudes, and perceptions of rural dog owners living in the districts of Gaza and Inhambane provinces, south of Mozambique, on cystic echinococcosis (CE) followed by a survey on the prevalence of gastrointestinal parasites with emphasis on Taeniidae infection in dogs from the same districts. A structured questionnaire was administered to 335 dog owners to assess their knowledge, attitudes and perceptions related to the risk factors for transmission, control, and prevention of CE. Responses were analyzed with SPSS software using the Chi-square test. To determine the prevalence of endoparasites, 723 dog fecal samples were collected and processed using a flotation technique to detect helminths/protozoa eggs/cysts/oocysts and modified Ziehl Neelsen staining to detect Cryptosporidium oocysts. Samples positive for taeniids were further processed to identify them to species level using molecular techniques. 3% (10/335) of respondents of the questionnaire were aware of CE which was higher in males (2.7%; 9/335) compared to females, in respondents aged 26-45 years old (2.1%; 7/335), and in agro-pastoralists (1.8%; 6/335). An overall prevalence of 93.7% (678/723) for at least one parasite species was found. Prevalence was 7.6% (55/723) for Spirocerca spp., 50.9% (368/723) for Ancylostoma spp., 4% (29/723) for Toxocara spp., 5.4% (39/723) for Trichuris spp., 8.7% (63/723) for Sarcocystis spp., 8.4% (61/723) for Dipylidium caninum, 8.7% (63/723) for Isospora spp., 2.2% (16/723) for Giardia spp. and 1.7% (12/723) for Cryptosporidium spp. Prevalence of taeniids was 2.4% (17/723) with six isolates identified as Taenia hydatigena and two as Taenia multiceps. Results of questionnaire survey showed that the Knowledge of cystic echinococcosis in districts of southern Mozambique was limited, and we recommend the development of targeted public health campaigns to raise awareness of local communities about transmission cycles and prevention CE. Future epidemiological studies should be conducted to investigate the potential source of CE infections in cattle by screening both wild and domestic canids.