Analytical Validation and Greenness Assessment of HPLC-DAD Method for Methylxanthines Analysis.

IF 1.2 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Omar J Portillo-Castillo, Paola Cobos-Cervantes, Norma C Cavazos-Rocha, Rocío Álvarez-Román, Luis Alejandro Pérez-López, Cecilia Delgado-Montemayor, Graciela Granados-Guzmán
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引用次数: 0

Abstract

In this study, a simple, and eco-friendly high-performance liquid chromatography-diode array detection (HPLC-DAD) method was developed and validated for analyzing methylxanthines, including theobromine (TB), theophylline (TF), and caffeine (CF). This method demonstrated excellent performance using a mobile phase of water and ethanol in a core-shell Kinetex® C18 column. The method was linear (1-100 µg mL-1), exhibiting high precision and accuracy with relative standard deviations below 2.67% and recoveries ranging from 98.17% to 108.23%. The detection and quantification limits ranged from 0.19-0.26 µg mL-1 and 0.64-0.87 µg mL-1, respectively. We applied this method to analyze powdered lemon-flavored beverages enriched with black or green tea extracts. CF was detected exceeding 1.62 g kg-1. The proposed method showed excellent greenness, evidenced by the Analytical Eco-Scale, Analytical GREEnness metric (AGREE) calculator, and ComplexGAPI.

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来源期刊
Acta Chimica Slovenica
Acta Chimica Slovenica 化学-化学综合
CiteScore
2.50
自引率
25.00%
发文量
80
审稿时长
1.0 months
期刊介绍: Is an international, peer-reviewed and Open Access journal. It provides a forum for the publication of original scientific research in all fields of chemistry and closely related areas. Reviews, feature, scientific and technical articles, and short communications are welcome.
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