Omar J Portillo-Castillo, Paola Cobos-Cervantes, Norma C Cavazos-Rocha, Rocío Álvarez-Román, Luis Alejandro Pérez-López, Cecilia Delgado-Montemayor, Graciela Granados-Guzmán
{"title":"Analytical Validation and Greenness Assessment of HPLC-DAD Method for Methylxanthines Analysis.","authors":"Omar J Portillo-Castillo, Paola Cobos-Cervantes, Norma C Cavazos-Rocha, Rocío Álvarez-Román, Luis Alejandro Pérez-López, Cecilia Delgado-Montemayor, Graciela Granados-Guzmán","doi":"10.17344/acsi.2024.8971","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, a simple, and eco-friendly high-performance liquid chromatography-diode array detection (HPLC-DAD) method was developed and validated for analyzing methylxanthines, including theobromine (TB), theophylline (TF), and caffeine (CF). This method demonstrated excellent performance using a mobile phase of water and ethanol in a core-shell Kinetex® C18 column. The method was linear (1-100 µg mL-1), exhibiting high precision and accuracy with relative standard deviations below 2.67% and recoveries ranging from 98.17% to 108.23%. The detection and quantification limits ranged from 0.19-0.26 µg mL-1 and 0.64-0.87 µg mL-1, respectively. We applied this method to analyze powdered lemon-flavored beverages enriched with black or green tea extracts. CF was detected exceeding 1.62 g kg-1. The proposed method showed excellent greenness, evidenced by the Analytical Eco-Scale, Analytical GREEnness metric (AGREE) calculator, and ComplexGAPI.</p>","PeriodicalId":7122,"journal":{"name":"Acta Chimica Slovenica","volume":"72 1","pages":"180-194"},"PeriodicalIF":1.2000,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Chimica Slovenica","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.17344/acsi.2024.8971","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, a simple, and eco-friendly high-performance liquid chromatography-diode array detection (HPLC-DAD) method was developed and validated for analyzing methylxanthines, including theobromine (TB), theophylline (TF), and caffeine (CF). This method demonstrated excellent performance using a mobile phase of water and ethanol in a core-shell Kinetex® C18 column. The method was linear (1-100 µg mL-1), exhibiting high precision and accuracy with relative standard deviations below 2.67% and recoveries ranging from 98.17% to 108.23%. The detection and quantification limits ranged from 0.19-0.26 µg mL-1 and 0.64-0.87 µg mL-1, respectively. We applied this method to analyze powdered lemon-flavored beverages enriched with black or green tea extracts. CF was detected exceeding 1.62 g kg-1. The proposed method showed excellent greenness, evidenced by the Analytical Eco-Scale, Analytical GREEnness metric (AGREE) calculator, and ComplexGAPI.
期刊介绍:
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