Chemical profile, antiproliferative and antioxidant activities of Genista januensis subsp. lydia (Boiss.) Kit Tan & Ziel (Fabaceae).

IF 1.2 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Merve Argon, Temine Sabudak, Buse Nur Yildirim, Bahadır Batar, Hakime Hulya Orak, Mehmet Yabas, Ayten Bostanci, Hilmican Caliskan
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引用次数: 0

Abstract

The aim of this study was to investigate the anticancer and antioxidant activities of the G. januensis subsp. lydia (Fabaceae) and to isolate the phytochemical compounds responsible for these activities. In this study, the G. januensis plant was extracted by maceration with increasing polarity and the anticancer activity of the crude extracts was investigated by performing cytotoxicity, apoptosis and cell cycle analyses.  In addition, the total phenolic/flavonoid content was determined in crude extracts and antioxidant activity. According to the results of anticancer and antioxidant activities, the EtOAc extract was found to have the highest activity and this extract was selected for an isolation study. All purified flavonoid compounds from the EtOAc extract were elucidated using 1D and 2D NMR and mass spectroscopy methods and compared with the literature data. A new biflavonoid (4) was isolated together with three known isoflavonoids (Genistein (1), Genistin (2) and 4'-hydroxy isoflavon (3)) from Genista januensis subsp. lydia (Fabaceae). MTT analysis of the compounds 2, 3, and 4 was utilized to evaluate the anticancer activity. Moreover, compound 4 had antioxidant activities determined. These findings suggest that G. januensis subsp. lydia has potential for further study as a natural source of antiproliferative and antioxidant agents.

姜草亚属的化学性质、抗增殖活性和抗氧化活性。丽迪雅(木香)。Kit Tan & Ziel(豆科)。
本研究的目的是研究紫金草亚种的抗癌和抗氧化活性。lydia (Fabaceae),并分离负责这些活性的植物化学化合物。本研究采用增加极性浸渍法提取黄芪,并通过细胞毒性、细胞凋亡和细胞周期分析研究其抗癌活性。此外,测定了粗提物中总酚/类黄酮含量及抗氧化活性。根据抗肿瘤和抗氧化活性的结果,发现EtOAc提取物具有最高的活性,并选择该提取物进行分离研究。采用1D、2D NMR和质谱方法对EtOAc提取物中所有纯化的类黄酮化合物进行了鉴定,并与文献数据进行了比较。从龙珠亚属中分离到一种新的双黄酮(4)和三种已知的异黄酮(染料木素(1)、染料木素(2)和4′-羟基异黄酮(3))。丽迪雅(蝶形花科)。利用MTT分析对化合物2、3、4的抗肿瘤活性进行评价。此外,化合物4还具有抗氧化活性。这些研究结果表明,januensis亚种。Lydia作为抗增殖和抗氧化剂的天然来源有进一步研究的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta Chimica Slovenica
Acta Chimica Slovenica 化学-化学综合
CiteScore
2.50
自引率
25.00%
发文量
80
审稿时长
1.0 months
期刊介绍: Is an international, peer-reviewed and Open Access journal. It provides a forum for the publication of original scientific research in all fields of chemistry and closely related areas. Reviews, feature, scientific and technical articles, and short communications are welcome.
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