The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan

IF 6.5 1区生物学 Q1 PLANT SCIENCES

Plant Physiology Pub Date : 2025-03-19 DOI:10.1093/plphys/kiaf096

Xueying Zhang, Shuting Zhang, Shuangjie Wang, Wentao Ma, Tingkai Zhai, Jie Gao, Chunwang Lai, Zihao Zhang, Yukun Chen, Zhongxiong Lai, Yuling Lin

{"title":"The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan","authors":"Xueying Zhang, Shuting Zhang, Shuangjie Wang, Wentao Ma, Tingkai Zhai, Jie Gao, Chunwang Lai, Zihao Zhang, Yukun Chen, Zhongxiong Lai, Yuling Lin","doi":"10.1093/plphys/kiaf096","DOIUrl":null,"url":null,"abstract":"Heat stress can seriously affect plant growth and development. Ethylene response factors (ERFs) play important roles in plant development and physiological responses. Here, we identified DlERF6, an ERF family transcription factor that promotes heat tolerance in Dimocarpus longan. DlERF6 was strongly induced by heat stress and IAA treatment in longan roots. Overexpression of DlERF6 generated abundant, fast-growing hairy roots and enhanced longan heat stress tolerance by promoting IAA biosynthesis and reactive oxygen species (ROS) scavenging. Additional assays indicated that DlERF6 directly binds to the DlGH3.5 promoter and represses its expression. Overexpressing DlGH3.5 reduced hairy root number, root length and heat tolerance, concomitant with a reduction in IAA content and ROS scavenging. Collectively, these results reveal the molecular mechanism through which the DlERF6–DlGH3.5 module regulates root growth and heat stress tolerance, providing a gene network that can be used for the genetic improvement of longan.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"183 1","pages":""},"PeriodicalIF":6.5000,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/plphys/kiaf096","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Heat stress can seriously affect plant growth and development. Ethylene response factors (ERFs) play important roles in plant development and physiological responses. Here, we identified DlERF6, an ERF family transcription factor that promotes heat tolerance in Dimocarpus longan. DlERF6 was strongly induced by heat stress and IAA treatment in longan roots. Overexpression of DlERF6 generated abundant, fast-growing hairy roots and enhanced longan heat stress tolerance by promoting IAA biosynthesis and reactive oxygen species (ROS) scavenging. Additional assays indicated that DlERF6 directly binds to the DlGH3.5 promoter and represses its expression. Overexpressing DlGH3.5 reduced hairy root number, root length and heat tolerance, concomitant with a reduction in IAA content and ROS scavenging. Collectively, these results reveal the molecular mechanism through which the DlERF6–DlGH3.5 module regulates root growth and heat stress tolerance, providing a gene network that can be used for the genetic improvement of longan.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Physiology 生物-植物科学

CiteScore

12.20

自引率

5.40%

发文量

535

审稿时长

2.3 months

期刊介绍： Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.