Deep-Penetrating and High-Resolution Continuous-Wave Nonlinear Microscopy Based on Homologous Dual-Emission Upconversion Adaptive Optics

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-03-20 DOI:10.1021/acs.nanolett.5c01030

Jing Yao, Zhipeng Yu, Yufeng Gao, Baoju Wang, Zhiyuan Wang, Tianting Zhong, Binxiong Pan, Huanhao Li, Hui Hui, Wei Zheng, Qiuqiang Zhan, Puxiang Lai

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引用次数: 0

Abstract

Lanthanide-doped upconversion nanoparticles (UCNPs) are emerging as innovative nonlinear probes in biomedical studies, offering the unique capability to simultaneously emit both visible (VIS) and near-infrared (NIR) photons under continuous-wave (CW) NIR excitation. However, deep-tissue high-resolution imaging remains challenging due to the trade-off between VIS emission (higher resolution, limited penetration) and NIR emission (deeper penetration, lower resolution). Here we present a CW nonlinear microscopy based on homologous dual-emission upconversion adaptive optics, leveraging Tm³⁺/Yb³⁺ co-doped UCNPs’ dual 455 nm/800 nm emission: the 800 nm emission for aberration measurement (guide-star) in deep tissues and the 455 nm emission for high-resolution imaging at matching depths. Using a home-built nonlinear laser scanning microscope with a 975 nm CW laser, we achieved near-diffraction-limited imaging (480 nm laterally) at a 500 μm depth in the mouse brain environment with significant optical aberrations. This strategy expands UCNPs’ applications and innovates the exploration of deep-tissue optical features.

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.