Prognostic value of neurotensin in experimental terminal ileitis.

IF 1.4 4区医学 Q4 ENGINEERING, BIOMEDICAL

Technology and Health Care Pub Date : 2025-03-01 Epub Date: 2024-12-01 DOI:10.1177/09287329241295867

Hongyu Zhou, Quan He, Rong Xiao, Yeqing Chen, Feifei Zhu, Haojuan Zhu, Mingwei Han, Guohua Zhou

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引用次数: 0

Abstract

BackgroundTerminal ileitis (TI) is an inflammatory condition of the distal portion of the ileum.ObjectiveTo investigate the changes and significance of neurotensin (NT) in the course of experimental terminal ileitis (ETI).MethodsA total of 90 Sprague Dawley (SD) rats were randomly divided into the control, model, and suture groups (30 rats in each group). After 2, 4, and 8 weeks of surgery, 10 TI tissues were taken for endoscopic observation in each group. Hematoxylin-eosin (HE) staining was used to detect the pathological changes. The NT levels in serum and terminal ileum mucosa were detected by Enzyme-linked immunosorbent assay (ELISA).ResultsAcute inflammatory changes were observed in the suture and the model groups after 2-week of operation. At 4 weeks, compared to the control group, the inflammatory damage in the model group became heavier, but it was reduced in the suture group. At 8 weeks, the model group showed chronic inflammation. However, there was no obvious inflammatory cell infiltration in both the suture group and control group. NT levels were increased in the suture and model groups at 2 weeks, particularly in the model group, and they were significantly higher in the suture and model groups than those in the control group (P < 0.05). At 4 weeks, the NT levels in the model group continued to rise, while they decreased in the suture group (P < 0.05). After 8 weeks, NT levels in the model group were significantly higher than those in the suture and control groups (P < 0.05). Furthermore, from 2 weeks to 8 weeks, the NT concentration in the model group gradually increased, and the suture group increased for 2 weeks, followed by a downward trend.Conclusions: In the ETI, the changes in NT concentration in serum and TI mucosa were positively correlated with the degree of inflammation.

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神经紧张素对实验性终末回肠炎的预后价值。

背景：末端回肠炎（TI）是一种发生在回肠远端部分的炎症。目的探讨实验性终末回肠炎（ETI）过程中神经紧张素（NT）的变化及其意义。方法90只SD大鼠随机分为对照组、模型组和缝合组，每组30只。术后2周、4周、8周，每组取TI组织10个进行内镜观察。采用苏木精-伊红（HE）染色检测病理变化。采用酶联免疫吸附试验（ELISA）检测血清及回肠末端黏膜NT水平。结果术后2周，缝合组和模型组均出现急性炎症变化。4周时，与对照组比较，模型组炎症损伤加重，而缝合组炎症损伤减轻。8周时，模型组出现慢性炎症。而缝线组和对照组均未见明显炎症细胞浸润。2周时，缝合组和模型组NT水平均升高，模型组尤以模型组明显高于对照组（P P P P）。结论：ETI大鼠血清及TI黏膜NT浓度变化与炎症程度呈正相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Technology and Health Care HEALTH CARE SCIENCES & SERVICES-ENGINEERING, BIOMEDICAL

CiteScore

2.10

自引率

6.20%

发文量

282

审稿时长

>12 weeks

期刊介绍： Technology and Health Care is intended to serve as a forum for the presentation of original articles and technical notes, observing rigorous scientific standards. Furthermore, upon invitation, reviews, tutorials, discussion papers and minisymposia are featured. The main focus of THC is related to the overlapping areas of engineering and medicine. The following types of contributions are considered: 1.Original articles: New concepts, procedures and devices associated with the use of technology in medical research and clinical practice are presented to a readership with a widespread background in engineering and/or medicine. In particular, the clinical benefit deriving from the application of engineering methods and devices in clinical medicine should be demonstrated. Typically, full length original contributions have a length of 4000 words, thereby taking duly into account figures and tables. 2.Technical Notes and Short Communications: Technical Notes relate to novel technical developments with relevance for clinical medicine. In Short Communications, clinical applications are shortly described. 3.Both Technical Notes and Short Communications typically have a length of 1500 words. Reviews and Tutorials (upon invitation only): Tutorial and educational articles for persons with a primarily medical background on principles of engineering with particular significance for biomedical applications and vice versa are presented. The Editorial Board is responsible for the selection of topics. 4.Minisymposia (upon invitation only): Under the leadership of a Special Editor, controversial or important issues relating to health care are highlighted and discussed by various authors. 5.Letters to the Editors: Discussions or short statements (not indexed).