Ascorbic acid exhibits more of a protective effect than estradiol against nephrotoxicity induced by malathion in rats: a histopathological and molecular docking study.

IF 1.2 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Turkish Journal of Medical Sciences Pub Date : 2024-10-29 eCollection Date: 2025-01-01 DOI:10.55730/1300-0144.5974
Mohammad Alhilal, Mahmoud Elsayed Mohamed Salem, Ahmed Ali Albakoush, Suzan Alhilal, Basant Farag, Sobhi M Gomha
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引用次数: 0

Abstract

Background/aim: Despite the known harmful effects associated with malathion toxicity in various organs, it continues to be widely used for plant protection and insect control. This study is the first to compare the protective effects of estradiol and ascorbic acid against malathion-induced nephrotoxicity through histopathological assessment and molecular docking analyses.

Materials and methods: This study was conducted using 20 female albino rats that were distributed into sham, malathion, malathion + estradiol, and malathion + ascorbic acid groups. Nephrotoxicity was induced by daily treatment with malathion and the effects of estradiol and ascorbic on nephrotoxicity were evaluated. After 4 weeks of treatment, the animals were sacrificed and the kidneys were examined following hematoxylin and eosin (H&E) staining. Histopathology results were supported by molecular docking studies of estradiol and ascorbic acid against a target protein (PDB ID: 2YMX), the peptide inhibitor Fab408 inhibiting acetylcholinesterase (AChE). The inhibition of AChE is the primary mechanism of the toxic effects of malathion.

Results: Histopathological examination revealed a notable elevation (p < 0.001) in degeneration and necrosis within the tubular epithelium and interstitial nephritis in the malathion group compared to the sham group. Daily administration of estradiol and ascorbic acid resulted in a notable reduction (p = 0.0022) in the severity of these histopathological changes in the malathion + estradiol and malathion + ascorbic acid groups compared to the malathion group. Of these, the most significant decreases were observed in the malathion + ascorbic acid group. Docking studies of these compounds against the selected protein (PDB ID: 2YMX) revealed promising binding scores. Ascorbic acid exhibited the highest docking score (-6.44 kcal/mol), indicating a favorable binding interaction with this protein.

Conclusion: Estradiol and ascorbic acid exert protective effects against malathion-induced nephrotoxicity, whereas ascorbic acid showed superior efficacy compared to estradiol. This result was further supported by molecular docking studies.

抗坏血酸对马拉硫磷引起的大鼠肾毒性表现出比雌二醇更多的保护作用:一项组织病理学和分子对接研究。
背景/目的:尽管已知马拉硫磷对各器官的毒性有害,但它仍被广泛用于植物保护和昆虫防治。本研究首次通过组织病理学评估和分子对接分析,比较了雌二醇和抗坏血酸对马拉硫磷所致肾毒性的保护作用。材料与方法:选用雌性白化大鼠20只,随机分为假药组、马拉硫磷组、马拉硫磷+雌二醇组、马拉硫磷+抗坏血酸组。每日给予马拉硫磷治疗引起肾毒性,并评价雌二醇和抗坏血酸对肾毒性的影响。治疗4周后处死大鼠,采用苏木精和伊红(H&E)染色检查肾脏。组织病理学结果得到了雌二醇和抗坏血酸对靶蛋白(PDB ID: 2YMX)、抑制乙酰胆碱酯酶(AChE)的肽抑制剂Fab408的分子对接研究的支持。对乙酰胆碱的抑制是马拉硫磷毒性作用的主要机制。结果:组织病理学检查显示,与假手术组相比,马拉硫磷组肾小管上皮变性、坏死及间质性肾炎明显升高(p < 0.001)。与马拉硫磷组相比,每日给予雌二醇和抗坏血酸导致马拉硫磷+雌二醇和马拉硫磷+抗坏血酸组这些组织病理学变化的严重程度显著降低(p = 0.0022)。其中,在马拉硫磷+抗坏血酸组观察到最显著的下降。这些化合物与选定蛋白(PDB ID: 2YMX)的对接研究显示出有希望的结合分数。抗坏血酸显示出最高的对接分数(-6.44 kcal/mol),表明与该蛋白有良好的结合相互作用。结论:雌二醇和抗坏血酸对马拉硫磷所致的肾毒性具有保护作用,抗坏血酸的作用优于雌二醇。分子对接研究进一步支持了这一结果。
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来源期刊
Turkish Journal of Medical Sciences
Turkish Journal of Medical Sciences 医学-医学:内科
CiteScore
4.60
自引率
4.30%
发文量
143
审稿时长
3-8 weeks
期刊介绍: Turkish Journal of Medical sciences is a peer-reviewed comprehensive resource that provides critical up-to-date information on the broad spectrum of general medical sciences. The Journal intended to publish original medical scientific papers regarding the priority based on the prominence, significance, and timeliness of the findings. However since the audience of the Journal is not limited to any subspeciality in a wide variety of medical disciplines, the papers focusing on the technical  details of a given medical  subspeciality may not be evaluated for publication.
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