Effect of temperature on the activity of efflux pumps in selected species of human opportunistic bacterial pathogens.

IF 2.5 4区医学 Q2 PARASITOLOGY

Memorias do Instituto Oswaldo Cruz Pub Date : 2025-03-17 eCollection Date: 2025-01-01 DOI:10.1590/0074-02760240162

Dusan Hurton, Lukás Hleba, Jana Petrová, Maros Laho, Ján Koren, Adriana Liptáková

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引用次数: 0

Abstract

Background: Efflux pumps represents one of the most important mechanisms of antibiotic resistance. They allow bacteria to expel antibiotics from their cells before they reach the target site.

Objectives: The main objective of this work was to examine how cultivation temperature and its variations affect the activity of efflux pumps in Acinetobacter junii, Bacillus cereus, and Enterobacter cloacae isolated from a skin swab.

Methods: The isolation and purification of bacterial colonies were done through the streak plate method. For the identification of bacterial species, MALDI-TOF was utilised. To detect the activity of efflux pumps, agar-ethidium bromide cartwheel method was implemented.

Findings: The accumulation of ethidium bromide (EtBr) in bacterial cells was higher at 43ºC than at 30ºC, so the activity of efflux pumps was reduced at 43ºC in all isolates. A temperature of 7ºC also caused increased cumulation of EtBr in the cells, hence decreasing the activity of efflux pumps in isolated bacteria. Moreover, B. cereus was more sensitive to meropenem at 43ºC than at 36ºC.

Main conclusions: The activity of efflux pumps and antibiotic resistance can be strongly affected by changes in incubation temperature in vitro in tested human opportunistic bacterial pathogens.

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温度对某些人类机会致病菌外排泵活性的影响。

背景：外排泵是抗生素耐药的重要机制之一。它们允许细菌在到达目标部位之前将抗生素从细胞中排出。目的：本研究的主要目的是研究培养温度及其变化如何影响从皮肤棉子中分离的朱尼不动杆菌、蜡样芽孢杆菌和阴沟肠杆菌的外排泵活性。方法：采用条纹板法分离纯化菌落。采用MALDI-TOF法进行菌种鉴定。为了检测外排泵的活性，采用了琼脂-溴化乙锭侧轮法。结果：43ºC时，细菌细胞中溴化乙锭（EtBr）的积累量高于30ºC，因此43ºC时所有分离株的外排泵活性均降低。7ºC的温度也会导致细胞中EtBr的积累增加，从而降低了分离细菌中外排泵的活性。蜡样芽孢杆菌在43℃时对美罗培南的敏感性高于36℃时。主要结论：被试人条件致病菌体外培养温度的变化对外排泵的活性和抗生素耐药性有很大影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Memorias do Instituto Oswaldo Cruz 医学-寄生虫学

CiteScore

5.00

自引率

3.60%

发文量

审稿时长

3-8 weeks

期刊介绍： Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.