Mitochondrial Dysfunction of the Subsynovial Connective Tissue in Patients With Carpal Tunnel Syndrome

IF 2.1 3区医学 Q2 ORTHOPEDICS

Journal of Orthopaedic Research® Pub Date : 2025-03-18 DOI:10.1002/jor.26064

Shuya Tanaka, Yutaka Mifune, Atsuyuki Inui, Kohei Yamaura, Takahiro Furukawa, Tatsuo Kato, Masaya Kusunose, Tomoyuki Matsumoto, Takehiko Matsushita, Ryosuke Kuroda

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引用次数: 0

Abstract

In idiopathic carpal tunnel syndrome (CTS), fibrosis and thickening of the subsynovial connective tissue (SSCT) increase pressure within the carpal tunnel, resulting in median nerve entrapment. Mitochondrial dysfunction in tissues reportedly leads to senescent cell accumulation and various diseases through reduced adenosine triphosphate (ATP) and excessive reactive oxygen species (ROS) production; however, no reports have linked this to CTS. Therefore, this study aimed to evaluate mitochondrial function in SSCTs of patients with CTS. This study investigated SSCTs obtained during carpal tunnel release surgery in patients with CTS (CTS group) and those obtained during tendon transfer or tendon rupture surgery in patients without CTS (control group) from April 2021 to March 2023 at our hospital. Outcome measures included superoxide dismutase (SOD) activity, gene expression levels, immunofluorescence staining, ATP production assays, and transmission electron microscopy (TEM). p values were calculated using the Mann–Whitney U test. The CTS and control groups included 10 and 5 patients (mean age, 67.8 ± 9.57 and 65.4 ± 9.75 years), respectively. The CTS group exhibited decreased SOD activity (p = 0.026), increased gene expression of mitochondrial biosynthetic factors, and decreased mitochondrial ATP production (p = 0.027). The CTS group showed increased mitochondrial ROS production (p = 0.038) on immunofluorescence and larger mitochondrial area (p = 0.030) and fewer mitochondrial cristae (p = 0.045) on TEM. Multiple mitochondrial function assays suggested mitochondrial dysfunction of SSCTs in the CTS group.

Statement of Clinical Significance

This research provided important information on the histological changes in the subsynovial connective tissue within the carpal tunnel in carpal tunnel syndrome.

Abstract Image

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腕管综合征患者滑膜下结缔组织的线粒体功能障碍。

在特发性腕管综合征（CTS）中，滑膜下结缔组织（SSCT）的纤维化和增厚增加了腕管内的压力，导致正中神经卡压。据报道，组织中的线粒体功能障碍通过减少三磷酸腺苷（ATP）和过量的活性氧（ROS）产生导致衰老细胞积累和各种疾病；然而，没有报道将此事与中旅联系起来。因此，本研究旨在评估CTS患者ssct的线粒体功能。本研究调查了我院于2021年4月至2023年3月期间，在有CTS患者（CTS组）的腕管松解手术中获得的SSCTs，以及在无CTS患者（对照组）的肌腱转移或肌腱断裂手术中获得的SSCTs。结果测量包括超氧化物歧化酶（SOD）活性、基因表达水平、免疫荧光染色、ATP生成测定和透射电子显微镜（TEM）。p值采用Mann-Whitney U检验计算。CTS组10例，对照组5例，平均年龄67.8±9.57岁，65.4±9.75岁。CTS组SOD活性降低（p = 0.026），线粒体生物合成因子基因表达增加，线粒体ATP生成减少（p = 0.027）。免疫荧光显示CTS组线粒体ROS生成增加（p = 0.038），透射电镜显示线粒体面积增大（p = 0.030），线粒体嵴减少（p = 0.045）。多项线粒体功能检测提示CTS组ssct存在线粒体功能障碍。临床意义：本研究为腕管综合征患者腕管滑膜下结缔组织组织学改变提供了重要信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Orthopaedic Research® 医学-整形外科

CiteScore

6.10

自引率

3.60%

发文量

261

审稿时长

3-6 weeks

期刊介绍： The Journal of Orthopaedic Research is the forum for the rapid publication of high quality reports of new information on the full spectrum of orthopaedic research, including life sciences, engineering, translational, and clinical studies.