Enhancing seed yield and nitrogen use efficiency of Brassica napus L. under low nitrogen by overexpression of G-proteins from Arabidopsis thaliana.

IF 5.6 2区 生物学 Q1 PLANT SCIENCES
Yuyu Xie, Yunyou Nan, Ayub Atif, Derong Shi, Hui Tian, Jing Hui, Yanfeng Zhang, Alan M Jones, Yajun Gao
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Abstract

Heterotrimeric G-proteins, composed of Gα, Gβ, and Gγ subunits, are involved in the regulation of multiple signaling pathways in plants. OsDEP1 (Gγ subunit-encoded protein) of rice and TaNBP1 (Gβ subunit-encoded protein) of wheat are homologs of Arabidopsis AGG3 and AGB1, respectively, which are regulators of grain size and also involved in nitrogen responses. However, the function of Arabidopsis G-proteins in nitrogen utilization under different nitrogen conditions has not been fully investigated. In this study, to evaluate the role of Arabidopsis G-proteins towards yield and nitrogen use efficiency (NUE), overexpressing transgenic lines AtGPA1, AtAGB1 together with AtAGG1 (AGB1-AGG1), with AtAGG2 (AGB1-AGG2), and with AtAGG3 (AGB1-AGG3) were created in the "K407" Brassica napus (B. napus). Analysis of multiple transgenic B. napus lines showed that overexpression of GPA1, AGB1-AGG1, AGB1-AGG2, or AGB1-AGG3 increased the biomass of seedling plants including a well-developed root system and increased nitrogen uptake under low and high nitrogen conditions. The activity of glutamine synthetase (GS), a key nitrogen assimilating enzyme, as well as the expression levels of genes that are involved in nitrogen uptake and assimilation were significantly increased in overexpressing plants under low nitrogen conditions. These properties enabled overexpressing plants to increase the number of seeds per silique by 12%-27% only under low nitrogen condition, effectively improving yield per plant by 9%-69% and NUE by 7%-49%. These results reveal roles of G-proteins in regulating seed traits and NUE, and provide a strategy that can substantially improve crop yield and NUE.

过表达拟南芥g蛋白提高低氮条件下甘蓝型油菜种子产量和氮素利用效率
异三聚体g蛋白由Gα、Gβ和Gγ亚基组成,参与植物多种信号通路的调控。水稻的OsDEP1 (Gγ亚基编码蛋白)和小麦的TaNBP1 (Gβ亚基编码蛋白)分别是拟南芥AGG3和AGB1的同源物,它们是籽粒大小的调节因子,也参与氮素响应。然而,在不同的氮素条件下,拟南芥g蛋白在氮素利用中的作用尚未得到充分的研究。为了评价拟南芥g蛋白对产量和氮素利用效率(NUE)的作用,本研究在“K407”甘蓝型油菜(B. napus)中建立了过表达AtGPA1、AtAGB1与AtAGG1 (AGB1-AGG1)、AtAGG2 (AGB1-AGG2)和AtAGG3 (AGB1-AGG3)的转基因株系。对多个转基因甘蓝型油菜株系的分析表明,在低氮和高氮条件下,过表达GPA1、AGB1-AGG1、AGB1-AGG2或AGB1-AGG3均能提高幼苗生物量,促进根系发育,提高氮素吸收量。低氮条件下,过表达植株的关键氮素同化酶谷氨酰胺合成酶(GS)活性以及参与氮素吸收和同化的基因表达量显著增加。这些特性使过表达植株在低氮条件下单株种子数可提高12% ~ 27%,单株产量可提高9% ~ 69%,氮肥利用效率可提高7% ~ 49%。这些结果揭示了g蛋白在调节种子性状和氮肥利用效率中的作用,为大幅度提高作物产量和氮肥利用效率提供了策略。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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