Evaluation of successive DNA extractions from cotton and flocked swabs.

IF 1.5 4区 医学 Q2 MEDICINE, LEGAL
Ella Pickell, Charlotte Arsenault, Kara L Jaremko, Georgiana Gibson-Daw, Deborah S B S Silva
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引用次数: 0

Abstract

The use of swabs to collect biological samples is a current practice in forensic investigations. Once a sample is collected, the DNA is extracted and used for testing at the crime lab. DNA extraction can often be sub-optimal in yield, and identifying new approaches to obtain larger DNA quantities from casework samples is critical. The goal of this study was to investigate the success rate of obtaining sufficient DNA yields from previously extracted swabs. Reference and case-type samples were collected using different types of swabs and genomic DNA was extracted from samples multiple times using only the QIAamp® DNA Investigator Kit or using this kit in combination with the Investigator Lyse&Spin Basket Kit. DNA samples were amplified using the GlobalFiler™ PCR Amplification Kit and separated on a SeqStudio Genetic Analyzer. All types of swabs and extraction methods yielded DNA after three extractions, and complete DNA profiles were produced for reference and case-type samples up to the third extraction. This provides a proof-of-concept that it is possible to recover DNA from previously extracted swabs in sufficient yield for full STR profiles. Also, trace DNA casework swabs can be extracted twice in succession and extracts pooled to increase yields for necessary DNA tests.

棉签和蜂群拭子连续DNA提取的评价。
使用棉签收集生物样本是目前法医调查的一种做法。一旦样本被收集,DNA就会被提取出来,并在犯罪实验室进行测试。DNA提取在产量上往往不是最优的,从案例样本中找到获得更大量DNA的新方法至关重要。本研究的目的是调查从先前提取的拭子中获得足够DNA产量的成功率。使用不同类型的拭子收集参考和病例型样本,仅使用QIAamp®DNA调查员试剂盒或将该试剂盒与调查员Lyse&Spin Basket试剂盒联合使用,从样本中多次提取基因组DNA。DNA样本使用GlobalFiler™PCR扩增试剂盒扩增,并在SeqStudio遗传分析仪上分离。三次提取后,所有类型的拭子和提取方法均可获得DNA,并在第三次提取前生成完整的DNA图谱供参考和病例型样本使用。这提供了一个概念验证,即从先前提取的拭子中以足够的产量恢复DNA以获得完整的STR谱是可能的。此外,痕量DNA样本可以连续提取两次,并将提取液汇集在一起,以增加必要DNA测试的产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Forensic Science, Medicine and Pathology
Forensic Science, Medicine and Pathology MEDICINE, LEGAL-PATHOLOGY
CiteScore
3.90
自引率
5.60%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Forensic Science, Medicine and Pathology encompasses all aspects of modern day forensics, equally applying to children or adults, either living or the deceased. This includes forensic science, medicine, nursing, and pathology, as well as toxicology, human identification, mass disasters/mass war graves, profiling, imaging, policing, wound assessment, sexual assault, anthropology, archeology, forensic search, entomology, botany, biology, veterinary pathology, and DNA. Forensic Science, Medicine, and Pathology presents a balance of forensic research and reviews from around the world to reflect modern advances through peer-reviewed papers, short communications, meeting proceedings and case reports.
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