Exposure of Bisphenols (BPA, BPB and BPC) in HepG2 Cells Results in Lysosomal Dysfunction and Lipid Accumulation.

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Cell Biology International Pub Date : 2025-03-18 DOI:10.1002/cbin.70017

Kiran Gill, Eshika Bindal, Parul Garg, Deepak Kumar, Rajasri Bhattacharyya, Dibyajyoti Banerjee

引用次数: 0

Abstract

Nonalcoholic fatty liver disease poses a significant public health concern, as do the issues surrounding plastic usage. The bisphenols are reported to cause fat accumulation in the liver. However, literature is scanty about the effect of bisphenols on lysosomes or lysosomal functions. We predicted the interaction of bisphenols with lysosomal proteins available in the online databases using in silico tools. Molecular docking revealed that chosen Bisphenols interact with critical lysosomal proteins including lipid hydrolyzing enzymes. Following exposure of BPA, BPB and BPC to HepG2 cells fat accumulation and lysosomal functions were evaluated. Exposure to BPB and BPC results intracellular fat accumulation under experimental conditions like BPA. All three Bisphenols disturb lysosomal homeostasis perhaps by different mechanisms. Overall our results suggest that Bisphenols can also cause fat accumulation in liver by disturbing lysosomal homeostasis.

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双酚（BPA、BPB 和 BPC）暴露于 HepG2 细胞会导致溶酶体功能紊乱和脂质积累。

非酒精性脂肪性肝病引起了重大的公共卫生问题，与塑料使用有关的问题也是如此。据报道，双酚类物质会导致肝脏脂肪堆积。然而，关于双酚类物质对溶酶体或溶酶体功能的影响，文献很少。我们预测了双酚与溶酶体蛋白的相互作用，可在在线数据库中使用计算机工具。分子对接显示，所选择的双酚类物质与包括脂质水解酶在内的关键溶酶体蛋白相互作用。BPA、BPB和BPC暴露于HepG2细胞后，对其脂肪积累和溶酶体功能进行了评价。在BPA等实验条件下，暴露于BPB和BPC会导致细胞内脂肪积累。这三种双酚类物质可能通过不同的机制扰乱溶酶体稳态。总的来说，我们的研究结果表明，双酚类物质也可以通过扰乱溶酶体稳态而引起肝脏脂肪堆积。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Biology International 生物-细胞生物学

CiteScore

7.60

自引率

0.00%

发文量

208

审稿时长

1 months

期刊介绍： Each month, the journal publishes easy-to-assimilate, up-to-the minute reports of experimental findings by researchers using a wide range of the latest techniques. Promoting the aims of cell biologists worldwide, papers reporting on structure and function - especially where they relate to the physiology of the whole cell - are strongly encouraged. Molecular biology is welcome, as long as articles report findings that are seen in the wider context of cell biology. In covering all areas of the cell, the journal is both appealing and accessible to a broad audience. Authors whose papers do not appeal to cell biologists in general because their topic is too specialized (e.g. infectious microbes, protozoology) are recommended to send them to more relevant journals. Papers reporting whole animal studies or work more suited to a medical journal, e.g. histopathological studies or clinical immunology, are unlikely to be accepted, unless they are fully focused on some important cellular aspect. These last remarks extend particularly to papers on cancer. Unless firmly based on some deeper cellular or molecular biological principle, papers that are highly specialized in this field, with limited appeal to cell biologists at large, should be directed towards journals devoted to cancer, there being very many from which to choose.