Adaptive evolution of cytochrome b in songbirds.

IF 1.8 4区 生物学 Q3 BIOLOGY
Biology Open Pub Date : 2025-03-19 DOI:10.1242/bio.061908
Hagai Rottenberg
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引用次数: 0

Abstract

The mitochondrial bc1 complex catalyzes the oxidation of ubiquinol by reducing cytochrome c. Cytochrome b, the catalytic core of bc1, generates superoxide during the oxidation of ubiquinol. Excessive superoxide production is known to accelerate aging and neurodegeneration. Songbirds (oscine, Passeri) exhibit lower production of mitochondrial ROS and greatly accelerated evolution of cytochrome b, relative to all other modern birds, suggesting adaptive selection for lower generation of ROS. Here we identified songbirds-specific substitutions in modern bird's cytochrome b amino-acids sequences and examined the high-resolution structures of the chicken bc1 complex in an effort to predict the effect of these substitutions on the function of bc1. Many of the songbirds-specific substitution cluster around sites that are critical for the function of bc1. One cluster of substitutions interacts with heme BH. A second cluster of substitutions interacts with residues in the ubiquinone reduction site, Qi. Both groups of substitution may affect the rate of reduction of ubiquinone at the Qi site. Another cluster of cytochrome b substitutions interacts with the hinge region of the Rieske protein that transfers electron from cytochrome b to cytochrome c1. These songbirds-specific substitutions appear to be selected to modulate the rate of both ubiquinol oxidation at the Qo site and ubiquinone reduction at the Qi site thereby modulating the rate of superoxide production. These findings are compatible with the hypothesis that cytochrome b evolution in songbirds was driven by selection of substitutions that reduce the rate of superoxide production thereby increasing songbird lifespan and cognitive abilities.

鸣禽细胞色素 b 的适应性进化。
线粒体bc1复合体通过还原细胞色素c来催化泛醇氧化,bc1的催化核心细胞色素b在泛醇氧化过程中产生超氧化物。过量的超氧化物会加速衰老和神经退化。与所有其他现代鸟类相比,鸣禽(oscine,雀鸟)线粒体ROS的产生较低,细胞色素b的进化大大加快,这表明了对低代ROS的适应性选择。我们在现代鸟类细胞色素b氨基酸序列中发现了鸣禽特异性的替换,并检测了鸡bc1复合体的高分辨率结构,以预测这些替换对bc1功能的影响。许多鸣禽特异性替代聚集在对bc1功能至关重要的位点周围。一个取代簇与血红素BH相互作用。第二簇取代与泛醌还原位点Qi中的残基相互作用。两组取代都可能影响Qi位点泛醌的还原速率。另一簇细胞色素b取代与Rieske蛋白的铰链区相互作用,将电子从细胞色素b转移到细胞色素c1。这些鸣禽特异性的取代似乎被选择来调节Qo位点的泛醇氧化速率和Qi位点的泛醇还原速率,从而调节超氧化物的产生速率。这些发现与鸣禽细胞色素b进化是由替代选择驱动的假设相一致,替代选择减少了超氧化物的产生速度,从而延长了鸣禽的寿命和认知能力。
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来源期刊
Biology Open
Biology Open BIOLOGY-
CiteScore
3.90
自引率
0.00%
发文量
162
审稿时长
8 weeks
期刊介绍: Biology Open (BiO) is an online Open Access journal that publishes peer-reviewed original research across all aspects of the biological sciences. BiO aims to provide rapid publication for scientifically sound observations and valid conclusions, without a requirement for perceived impact.
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