Intraluminal pressure triggers a rapid and persistent reinforcement of endothelial barriers.

IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-03-18 DOI:10.1039/d5lc00104h
Aurélien Bancaud, Tadaaki Nakajima, Jun-Ichi Suehiro, Baptiste Alric, Florent Morfoisse, Jean Cacheux, Yukiko T Matsunaga
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引用次数: 0

Abstract

In response to mechanical cues, endothelial cells elicit highly sensitive cellular response pathways that contribute to the regulation of the physiology and disorders of the vascular system. However, it remains relatively unexplored how endothelial tissues process and integrate the intraluminal pressure, and in turn regulate the permeation flow across the vessel wall. Leveraging a tissue engineering approach to create microvessels (MVs), we measured real-time permeation flow induced by intraluminal pressures ranging from 0.1 to 2.0 kPa. Our findings reveal that mechanically stimulated MVs strengthen their barrier function within seconds of exposure to pressures below 1 kPa, with this enhanced barrier function persisting for 30 minutes. We demonstrate that this barrier reinforcement is linked to the closure of paracellular gaps. Additionally, we observe that it is associated with, and depends on, actin cytoskeleton reorganization, including the accumulation of stress fibers near intercellular junctions and the broadening of adherence junction protein localization. These findings provide insights into the ability of endothelial tissues to regulate interstitial fluid flow in response to sudden increases in blood pressure.

内皮细胞在对机械信号做出反应时,会引发高度敏感的细胞反应途径,这些途径有助于调节血管系统的生理和失调。然而,内皮组织如何处理和整合腔内压力,进而调节血管壁上的渗透流,这一点相对来说仍有待探索。我们利用组织工程方法创建了微血管(MVs),测量了腔内压力在 0.1 至 2.0 千帕范围内诱导的实时渗透流。我们的研究结果表明,受到机械刺激的微血管在承受低于 1 kPa 的压力后几秒钟内就能增强其屏障功能,这种增强的屏障功能可持续 30 分钟。我们证明,这种屏障强化与细胞旁间隙的闭合有关。此外,我们还观察到它与肌动蛋白细胞骨架重组有关,并且依赖于肌动蛋白细胞骨架重组,包括应力纤维在细胞间连接附近的聚集以及粘附连接蛋白定位的扩大。这些发现为了解内皮组织在血压突然升高时调节间质流体流动的能力提供了启示。
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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