The ZjMYB44-ZjPOD51 module enhances jujube defence response against phytoplasma by upregulating lignin biosynthesis

IF 8.7 1区农林科学 Q1 Agricultural and Biological Sciences

Horticulture Research Pub Date : 2025-03-19 DOI:10.1093/hr/uhaf083

Liman Zhang, Hongtai Li, Ximeng Wei, Yuanyuan Li, Zhiguo Liu, Mengjun Liu, Weijie Huang, Huibin Wang, Jin Zhao

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引用次数: 0

Abstract

Lignin is a major component of the plant cell wall and has a conserved basic defence function in higher plants, helping the plants cope with pathogen infection. However, the regulatory mechanism of lignin biosynthesis in plants under phytoplasma stress remains unclear. In this study, we reported that peroxidase 51 (ZjPOD51), which is involved in lignin monomer polymerization, was induced by phytoplasma infection and that overexpression of ZjPOD51 in phytoplasma-infected jujube seedlings and Arabidopsis plants significantly increased their defence response against phytoplasma. Yeast one-hybrid (Y1H) and luciferase (LUC) assays showed that ZjPOD51 transcription was directly upregulated by ZjMYB44. Genetic validation demonstrated that ZjMYB44 expression was also induced by phytoplasma infection and contributed to lignin accumulation, which consequently enhanced phytoplasma defence in a ZjPOD51-dependent manner. These results demonstrated that the ZjMYB44-ZjPOD51 module enhanced the jujube defence response against phytoplasma by upregulating lignin biosynthesis. Overall, our study first elucidates how plants regulate lignin to enhance their defence response against phytoplasma and provides clues for jujube resistance breeding.

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ZjMYB44-ZjPOD51模块通过上调木质素的生物合成来增强红枣对植物原体的防御反应

木质素是植物细胞壁的主要成分，在高等植物中具有保守的基本防御功能，帮助植物应对病原体感染。然而，植物原体胁迫下植物木质素生物合成的调控机制尚不清楚。本研究报道了参与木质素单体聚合的过氧化物酶51 （ZjPOD51）在植物原体感染后被诱导表达，并且ZjPOD51在植物原体感染的枣树和拟南芥幼苗中过表达可显著提高其对植物原体的防御反应。酵母单杂交（Y1H）和荧光素酶（LUC）分析表明，ZjMYB44直接上调ZjPOD51的转录。遗传验证表明，ZjMYB44的表达也受植原体感染诱导，并促进木质素积累，从而以zjpod51依赖的方式增强植原体防御。这些结果表明，ZjMYB44-ZjPOD51模块通过上调木质素的生物合成来增强红枣对植原体的防御反应。本研究首次阐明了植物如何调控木质素增强对植物原体的防御反应，为红枣抗病育种提供了线索。

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来源期刊

Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

11.20

自引率

6.90%

发文量

367

审稿时长

20 weeks

期刊介绍： Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.