Chemical Characterization and Anti-Obesity Activity of Exopolysaccharide Isolated from Scleroderma areolatum (Agaricomycetes).

International journal of medicinal mushrooms Pub Date : 2025-01-01 DOI:10.1615/IntJMedMushrooms.2025058054

Jiafeng Bai, Xiaoqin Peng, Yi Zhou, Zhizhong Hu, Changtong Lu, Xuewei Jia, Chunping Xu

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Abstract

Two polysaccharide fractions were successfully isolated from the crude exopolysaccharide (EPS) of Scleroderma areolatum via gel filtration chromatography. Size exclusion chromatography/multi-angle laser light scattering (SEC/MALLS) analysis showed that the weight of average molecular weights (Mw) of these fractions was 3.162 ȕ 106 (Fr-I) and 2.613 ȕ 106 (Fr-II). Both EPS fractions exhibited a compact globular structure in aqueous solution. Monosaccharide composition analysis revealed that they were primarily composed of mannose and glucuronic acid. FT-IR spectral analysis identified prominent functional groups, such as hydroxyl and carboxylic, typical of heteropolysaccharides. When administered as supplements in high-fat diets to obese rats for 8 weeks, the EPS fractions reduced body weight, serum inflammatory factor levels, and significantly regulated serum lipid levels. Additionally, they increased the total concentration of short-chain fatty acids (SCFA) in colonic digesta. These results suggest a potential role for EPS in mitigating obesity and related metabolic disorders in high-fat diet-induced obese rats.

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乳状硬皮病（菌类）胞外多糖的化学特性及抗肥胖活性研究。

采用凝胶过滤层析法，成功地从乳状硬皮病粗多糖（EPS）中分离出两个多糖组分。粒径排除色谱/多角度激光散射（SEC/MALLS）分析表明，这些馏分的平均分子量（Mw）分别为3.162°106 （Fr-I）和2.613°106 （Fr-II）。两种EPS馏分在水溶液中均呈现致密的球状结构。单糖组成分析表明，它们主要由甘露糖和葡萄糖醛酸组成。傅里叶变换红外光谱分析发现了突出的官能团，如羟基和羧基，是典型的杂多糖。当肥胖大鼠在高脂肪饮食中补充EPS时，8周后，EPS组分降低了体重，血清炎症因子水平，并显著调节了血脂水平。此外，它们还增加了结肠食糜中短链脂肪酸（SCFA）的总浓度。这些结果表明，EPS在减轻高脂肪饮食诱导的肥胖大鼠的肥胖和相关代谢紊乱方面具有潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International journal of medicinal mushrooms

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