Synergistic Effects of Hypoxia-Preconditioned Mesenchymal Stem Cells Secretome and Alkaline Water in Alleviating Oxidative Stress and Inflammation in Type 2 Diabetic Rats.

Materia socio-medica Pub Date : 2025-01-01 DOI:10.5455/msm.2024.37.4-10

Irni Nasution, Agung Putra, Eko Setiawan

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Abstract

Background: Chronic inflammation and oxidative stress are central to the pathophysiology of Type 2 Diabetes Mellitus (T2DM), contributing to the progression of metabolic dysfunction and related complications.

Objective: The aim of this study was to explore the therapeutic potential of combining hypoxia-preconditioned mesenchymal stem cell SH-MSC with alkaline water in a T2DM rat model.

Methods: T2DM was induced in Wistar rats through a high-fat diet (HFD) followed by streptozotocin (STZ) administration. A total of 30 healthy male Wistar rats were randomly assigned to five groups: healthy control, T2DM, T2DM + Metformin, T2DM + SH-MSC, and T2DM + SH-MSC + alkaline water.

Results: The combination of SH-MSC and alkaline water significantly reduced malondialdehyde (MDA) levels, a key indicator of lipid peroxidation, and suppressed the expression of p65 mRNA, a crucial component of the NF-κB signaling pathway. Notably, the most pronounced reduction in p65 mRNA expression was observed in the group receiving both SH-MSC and alkaline water, suggesting a synergistic effect in mitigating oxidative stress and inflammation.

Conclusion: These findings highlight the potential of SH-MSC and alkaline water as a novel therapeutic strategy for alleviating T2DM.

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背景：慢性炎症和氧化应激是2型糖尿病（T2DM）病理生理学的核心，导致代谢功能障碍和相关并发症的进展：本研究旨在探讨缺氧修复间充质干细胞 SH-MSC 与碱性水在 T2DM 大鼠模型中的治疗潜力：方法：通过高脂饮食（HFD）诱导Wistar大鼠T2DM，然后给予链脲佐菌素（STZ）。将 30 只健康雄性 Wistar 大鼠随机分为五组：健康对照组、T2DM 组、T2DM + 二甲双胍组、T2DM + SH-MSC 组和 T2DM + SH-MSC + 碱水组：结果：SH-间充质干细胞和碱性水的组合能显著降低丙二醛（MDA）水平（脂质过氧化的关键指标），并抑制 p65 mRNA（NF-κB 信号通路的重要组成部分）的表达。值得注意的是，在同时接受 SH-MSC 和碱性水的组别中，p65 mRNA 的表达量减少最为明显，这表明在减轻氧化应激和炎症方面存在协同效应：这些发现凸显了 SH-MSC 和碱性水作为缓解 T2DM 的新型治疗策略的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Materia socio-medica

CiteScore

1.80

自引率

0.00%

发文量