The role and diagnostics of miR-181d-5p in polycystic ovary syndrome in insulin-resistant women.

Abstract

Introduction: To promote the research progress of polycystic ovary syndrome (PCOS), this study analysed the role of micro RNA (miRNA) in PCOS, to provide new ideas for the development of PCOS.

Material and methods: A total of 101 PCOS patients and 95 healthy controls (HC) were enrolled in this study, and according to body mass index (BMI), the subjects were divided into overweight (ow) and non-overweight (non-ow), and analyses were performed to compare the statistical differences of clinically related indexes in each group. Serum miR-181d-5p expression was determined by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), and the diagnostic capability of miR-181d-5p for PCOS ow and non-ow was evaluated by receiver operating characteristic curve (ROC). The effect of miR-181d-5p on PCOS combined with insulin resistance (PCOS-IR) was analysed by multivariate logistic regression. The effects of miR-181d-5p on KGN cells under insulin resistance (IR) were analysed by proliferation and apoptosis experiments.

Results: In both ow and non-ow populations, miR-181d-5p expression was higher in PCOS patients than in the HC. miR-181d-5p can be a better aid in the diagnosis of PCOS ow and non-ow. miR-181d-5p can be used as a risk predictor of PCOS-IR (p = 0.014). It was highly expressed in PCOS-IR and had high predictive ability [area under the curve (AUC): 0.898] with high specificity (83.33%) and sensitivity (82.93%) in assisting the diagnosis of PCOS-IR. IR-inhibited proliferated and induced apoptosis of KGN cells, which was improved by miR-181d-5p downregulation.

Conclusions: This study proposed the role of miR-181d-5p in PCOS for the first time, providing a new reference for improving PCOS-IR.