Identification and Genome Characterization of Begomovirus and Satellite Molecules Associated with Lettuce (Lactuca sativa L.) Leaf Curl Disease.

IF 4 2区生物学 Q1 PLANT SCIENCES

Plants-Basel Pub Date : 2025-03-04 DOI:10.3390/plants14050782

Yafei Tang, Mengdan Du, Zhenggang Li, Lin Yu, Guobing Lan, Shanwen Ding, Tahir Farooq, Zifu He, Xiaoman She

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引用次数: 0

Abstract

Lettuce (Lactuca sativa L.) plants showing leaf curl and vein enation symptoms were found in Yunnan province, China. PCR detection with genus-specific primers revealed that symptomatic lettuce plants were infected with Begomovirus. The full-length viral component and satellite molecules were obtained by RCA, restriction enzyme digestion, PCR, cloning and DNA sequencing. A viral component (YN-2023-WJ) and three satellite molecules (YN-2023-WJ-alpha1, YN-2023-WJ-alpha2 and YN-2023-WJ-beta) were obtained from diseased lettuce plants. YN-2023-WJ exhibited the highest nt identity at 97.1% with pepper leaf curl Yunnan virus isolated from cigar plants. YN-2023-WJ-beta displayed the highest nt identity at 93.9% with tomato leaf curl China betasatellite. YN-2023-WJ-alpha1 showed the highest nt identity at 94.7% with ageratum yellow vein alphasatellite. YN-2023-WJ-alpha2 shared the highest nt identity at 75.6% with gossypium mustelinum symptomless alphasatellite and vernonia yellow vein Fujian alphasatellite. Based on the threshold for the classification of Begomovirus, Betasatellite and Alphasatellite, YN-2023-WJ was designated as a new isolate of PepLCYnV, YN-2023-WJ-beta as a new isolate of ToLCCNB and YN-2023-WJ-alpha1 as a new member of AYVA, whereas YN-2023-WJ-alpha2 was identified as a new geminialphasatellite species, for which the name pepper leaf curl Yunnan alphasatellite (PepLCYnA) is proposed. To the best of our knowledge, this is the first report of L. sativa L. infection by PepLCYnV associated with ToLCCNB, AYVA and PepLCYnA, and L. sativa L. is a new host plant of Begomovirus.

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中国云南省的莴苣（Lactuca sativa L.）植株出现了卷叶和叶脉枯萎症状。使用属特异性引物进行 PCR 检测发现，出现症状的莴苣植株感染了 Begomovirus 病毒。通过 RCA、限制性酶消化、PCR、克隆和 DNA 测序获得了全长病毒成分和卫星分子。从莴苣病株中获得了一个病毒成分（YN-2023-WJ）和三个卫星分子（YN-2023-WJ-alpha1、YN-2023-WJ-alpha2 和 YN-2023-WJ-beta）。YN-2023-WJ 与从雪茄植株中分离出的云南辣椒卷叶病毒的 nt 一致度最高，达到 97.1%。YN-2023-WJ-beta与番茄卷叶病毒中国β卫星的最高nt同一性为93.9%。YN-2023-WJ-alpha1 与 ageratum 黄脉碱基卫星的 nt 相同度最高，为 94.7%。YN-2023-WJ-alpha2 与棉铃虫无症状优势卫星和马铃薯黄脉福建优势卫星的 nt 一致度最高，为 75.6%。根据 Begomovirus、Beta 卫星和 Alphasatellite 的分类阈值，YN-2023-WJ 被定为 PepLCYnV 的新分离株，YN-2023-WJ-beta 被定为 ToLCCNB 的新分离株，YN-2023-WJ-alpha1 被定为 AYVA 的新成员、而YN-2023-WJ-alpha2则被鉴定为一种新的geminialphasatellite物种，并建议将其命名为辣椒卷叶云南αphasatellite（PepLCYnA）。据我们所知，这是首次报道 PepLCYnV 与 ToLCCNB、AYVA 和 PepLCYnA 相关联感染 L. sativa L.，L. sativa L.是 Begomovirus 的新宿主植物。

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来源期刊

Plants-Basel Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

6.50

自引率

11.10%

发文量

2923

审稿时长

15.4 days

期刊介绍： Plants (ISSN 2223-7747), is an international and multidisciplinary scientific open access journal that covers all key areas of plant science. It publishes review articles, regular research articles, communications, and short notes in the fields of structural, functional and experimental botany. In addition to fundamental disciplines such as morphology, systematics, physiology and ecology of plants, the journal welcomes all types of articles in the field of applied plant science.