Functional Analysis of the ZmPR5 Gene Related to Resistance Against Fusarium verticillioides in Maize.

IF 4 2区生物学 Q1 PLANT SCIENCES

Plants-Basel Pub Date : 2025-02-28 DOI:10.3390/plants14050737

Wei Yang, Hongyu Cai, Yuanqi Zhang, Junheng Hao, Yaqi Ma, Qinxuan He, Kun Zhao, Honggang Li, Ayue Fang, Dalong Hou, Xuejiao Ren

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Abstract

In this study, the gene ZmPR5, associated with resistance to ear rot, was identified through transcriptome data analysis of the maize inbred line J1259. The gene was subsequently cloned and its function was investigated. The ZmPR5 gene comprises an open reading frame of 525 base pairs, encoding a protein of 175 amino acids. ZmPR5 was overexpressed in Arabidopsis and the ZmPR5EMS mutant in maize, and they were subjected to q-PCR and measurements of antioxidant enzyme activities (POD, SOD, CAT, MDA), electrical conductivity, and chlorophyll content. The results indicate that the expression of ZmPR5 is up-regulated upon infection by Fusarium verticillioides, with significant differences observed in the activities of POD, SOD, CAT, MDA, electrical conductivity, and chlorophyll content. The study found that ZmPR5 is localized in the nucleus and interacts with Zm00001d020492 (WRKY53) and Zm00001d042140 (glucA). Trypan blue staining revealed that the stained area in the ZmPR5EMS mutant of maize was significantly larger than that in B73. The expression of ZmPR5 is closely associated with resistance to maize ear rot.

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玉米抗黄萎病相关基因ZmPR5的功能分析

本研究通过对玉米自交系J1259的转录组数据分析，鉴定了与抗穗腐病相关的基因ZmPR5。随后克隆了该基因并对其功能进行了研究。ZmPR5基因包括一个525个碱基对的开放阅读框，编码175个氨基酸的蛋白质。ZmPR5在拟南芥和玉米突变体ZmPR5EMS中过表达，并对它们进行了q-PCR和抗氧化酶活性（POD、SOD、CAT、MDA）、电导率和叶绿素含量的测定。结果表明，ZmPR5在黄萎病镰刀菌侵染后表达上调，在POD、SOD、CAT、MDA活性、电导率和叶绿素含量等方面存在显著差异。研究发现，ZmPR5定位于细胞核内，与Zm00001d020492 （WRKY53）和Zm00001d042140 （glucA）相互作用。台锥蓝染色显示，玉米ZmPR5EMS突变体的染色面积显著大于B73。ZmPR5的表达与玉米穗腐病抗性密切相关。

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来源期刊

Plants-Basel Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

6.50

自引率

11.10%

发文量

2923

审稿时长

15.4 days

期刊介绍： Plants (ISSN 2223-7747), is an international and multidisciplinary scientific open access journal that covers all key areas of plant science. It publishes review articles, regular research articles, communications, and short notes in the fields of structural, functional and experimental botany. In addition to fundamental disciplines such as morphology, systematics, physiology and ecology of plants, the journal welcomes all types of articles in the field of applied plant science.