Comparison of Two Bacillus Strains Isolated from the Coastal Zone in Barley (Hordeum vulgare L.) Under Salt Stress.

Abstract

Salt stress is one of the most important abiotic stress factors that negatively affects sustainable crop production, agricultural productivity, and microbial life. Increasing salt stress negatively affects the growth and development of barley, posing a threat to global food security. It is now known that inoculation of plant growth-promoting rhizobacteria (PGPR) has significant potential in increasing stress tolerance and yield in agricultural products. This study focused on the effects of Bacillus cereus CUN6 and Bacillus thuringiensis SIRB2, isolated from the coastal zone and tested for their PGPR capacities, on physiological (root length, shoot length, biomass, dry weight) and biochemical (total chlorophyll, total protein, hydrogen peroxide, lipid peroxidation, peroxidase activity (POX), catalase activity (CAT)) analyses in Hordeum vulgare L. seedlings under salt stress. The results showed that the two bacterial inoculations alleviated the negative effects of salt stress by increasing the root-shoot length, biomass, dry weight, chlorophyll content, and total protein content in barley plants. However, B.thuringiensis increased growth and development especially in root length, biomass, and dry weight compared to B.cereus. On the other hand, B.cereus significantly increased root length, biomass, and chlorophyll content under salt stress; these increases were 17%, 5%, and 7%, respectively. B.thuringiensis chlorophyll content increased by 4% in 300 mM NaCl compared to the control. When compared in terms of the antioxidant defense system, B.thuringiensis inoculation was more effective on CAT activity, while B.cereus inoculation was more effective on POX activity. Under salt stress, B.cereus and B.thuringiensis inoculation significantly decreased H₂O₂ content in barley; these decreases were 16% and 10%, respectively. Additionally, TBARs content was significantly decreased by B.cereus and B.thuringiensis inoculation under salt stress; these decreases were determined as 8% and 9%, respectively, compared to the control. These results indicated that both bacterial inoculations can alleviate the salt tolerance of barley seedlings by regulating antioxidant metabolism. This research focused on the potential of B.cereus and B.thuringiensis as biofertilizers against salt stress in barley based on physiological and biochemical analysis.