A new class of type VI secretion system effectors can carry two toxic domains and are recognized through the WHIX motif for export.

IF 9.8 1区生物学 Q1 Agricultural and Biological Sciences

PLoS Biology Pub Date : 2025-03-17 eCollection Date: 2025-03-01 DOI:10.1371/journal.pbio.3003053

Chaya Mushka Fridman, Kinga Keppel, Vladislav Rudenko, Jon Altuna-Alvarez, David Albesa-Jové, Eran Bosis, Dor Salomon

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引用次数: 0

Abstract

Gram-negative bacteria employ the type VI secretion system (T6SS) to deliver toxic effectors into neighboring cells and outcompete rivals. Although many effectors have been identified, their secretion mechanism often remains unknown. Here, we describe WHIX, a domain sufficient to mediate the secretion of effectors via the T6SS. Remarkably, we find WHIX in T6SS effectors that contain a single toxic domain, as well as in effectors that contain two distinct toxic domains fused to either side of WHIX. We demonstrate that the latter, which we name double-blade effectors, require two cognate immunity proteins to antagonize their toxicity. Furthermore, we show that WHIX can be used as a chassis for T6SS-mediated secretion of multiple domains. Our findings reveal a new class of polymorphic T6SS cargo effectors with a unique secretion domain that can deploy two toxic domains in one shot, possibly reducing recipients' ability to defend themselves.

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一类新的VI型分泌系统效应物可以携带两个有毒结构域，并通过WHIX基元识别出口。

革兰氏阴性菌利用VI型分泌系统（T6SS）将毒性效应物传递到邻近细胞中，从而战胜对手。虽然已经确定了许多效应物，但它们的分泌机制往往仍不清楚。在这里，我们描述了WHIX，一个足以通过T6SS介导效应物分泌的结构域。值得注意的是，我们在T6SS效应器中发现了WHIX，这些效应器包含一个单一的有毒结构域，以及包含两个不同的毒性结构域融合到WHIX的任何一侧。我们证明，后者，我们称之为双刃效应，需要两个同源免疫蛋白拮抗其毒性。此外，我们发现WHIX可以作为t6ss介导的多个结构域分泌的基础。我们的研究结果揭示了一类新的多态T6SS货物效应物，具有独特的分泌结构域，可以在一次注射中部署两个有毒结构域，可能降低受体自我防御的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PLoS Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOLOGY

CiteScore

15.40

自引率

2.00%

发文量

359

审稿时长

3-8 weeks

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