Cryosectioning and Immunostaining of Mouse Retina.

IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Yongqiong Lin, Yingjie Tong, Tongdan Zou, Jiajia Wang, Houbin Zhang
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引用次数: 0

Abstract

Tissue sectioning and immunohistochemistry are essential techniques in histological and pathological studies of retinal diseases using animal models. These methods enable detailed examinations of tissue morphologies and the localization of specific proteins within the tissue, which provide valuable insights into disease processes and mechanisms. Mice are the most widely used model for this purpose. However, because mouse eyeballs are small and mouse retinas are extremely delicate tissues, obtaining high-quality retinal sections and immunostaining images from mouse eyeballs is typically challenging. This study describes an improved protocol for cryosectioning mouse retinas and performing immunohistochemistry. An essential point of this protocol involves coating the eyeball with a layer of super glue, which prevents deformation of the eyeballs during the processes of cornea removal, lens extraction, and embedding. This step ensures the integrity of retinal morphologies is well preserved. This protocol highlights critical technical considerations and optimization strategies for consistently producing high-quality retinal sections and achieving excellent immunostaining results.

小鼠视网膜冷冻切片及免疫染色。
组织切片和免疫组织化学是视网膜疾病动物模型组织学和病理学研究的基本技术。这些方法能够详细检查组织形态和组织内特定蛋白质的定位,从而为疾病过程和机制提供有价值的见解。小鼠是用于此目的的最广泛的模型。然而,由于小鼠眼球很小,而小鼠视网膜是极其脆弱的组织,因此从小鼠眼球中获得高质量的视网膜切片和免疫染色图像通常具有挑战性。本研究描述了一个改进的方案冷冻切片小鼠视网膜和执行免疫组织化学。该方案的要点是在眼球上涂上一层强力胶,以防止眼球在角膜摘除、晶状体取出和植入过程中变形。这一步骤确保了视网膜形态学的完整性得到很好的保存。该方案强调了关键的技术考虑和优化策略,以始终如一地生产高质量的视网膜切片并获得出色的免疫染色结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Jove-Journal of Visualized Experiments
Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-
CiteScore
2.10
自引率
0.00%
发文量
992
期刊介绍: JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.
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