Frozen semen quality of Pasundan bulls with different individual variations.

IF 0.9 Q3 VETERINARY SCIENCES

Open Veterinary Journal Pub Date : 2025-01-01 Epub Date: 2025-01-31 DOI:10.5455/OVJ.2024.v15.i1.22

Abdullah Baharun, Pirda Parida Permadani Pertiwi, Annisa Rahmi, Natashya Chandra Rachmadanti, Ristika Handarini, Hikmayani Iskandar, Daud Samsudewa, Tulus Maulana, Syahruddin Said, Imam Darussalam, Nurcholis Nurcholis, Raden Iis Arifiantini

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引用次数: 0

Abstract

Background: Pasundan bulls are one of Indonesia's indigenous cattle breeds. The quality of frozen semen is essential for the success of artificial insemination (AI), as individual variations in parameters such as sperm motility, viability, and membrane integrity greatly influence reproductive outcomes.

Aim: The objective of this study was to evaluate the quality of frozen semen from Pasundan bulls with different individual variations.

Methods: Frozen semen from eight Pasundan bulls was obtained from the West Java Regional Artificial Insemination Center. Sperm motility was assessed using computer-assisted sperm analysis (CASA; SpermVision®, Germany). Sperm viability and abnormalities were determined by eosin-nigrosine staining. Plasma membrane integrity was evaluated using the hypoosmotic swelling test, acrosome integrity was assessed using fluorescein isothiocyanate-propidium iodide, and protamine deficiency was measured using chromomycin A3 fluorescence staining.

Results: Significant individual variations (p < 0.05) were found across all parameters of frozen semen quality, except for sperm abnormalities, which showed no significant differences (p > 0.05).

Conclusion: The results showed that individual variations significantly influence all quality parameters of frozen semen from Pasundan bulls, except for sperm abnormalities. Therefore, a thorough assessment of each bull is crucial to ensure the production of quality frozen semen.

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来源期刊

Open Veterinary Journal VETERINARY SCIENCES-

CiteScore

1.40

自引率

0.00%

发文量

112

审稿时长

12 weeks

期刊介绍： Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.