The complete mitochondrial genome of medicinally important wood-decaying fungus Tyromyces fissilis within the family Incrustoporiaceae, Polyporales.

Abstract

Tyromyces fissilis (Berk. & M.A.Curtis) Donk 1933, a globally renowned white-rot basidiomycete belonging to the Polyporales order, holds significant potential for lignin degradation, yet its mitochondrial genome has received comparatively little attention. Our study concentrates on a specimen designated T. fissilis NEFU_01, sourced from the Forest Botanical Garden in Heilongjiang Province, China. Utilizing next-generation sequencing (NGS) technology, we have successfully delineated the complete mitochondrial genome of this T. fissilis isolate. The genome is composed of 15 protein-coding genes (PCGs), an array of 24 transfer RNAs (tRNAs), and a pair of ribosomal RNAs (rRNAs), encompassing a total of 163,380 base pairs (bp). Additionally, the genome encodes 28 LAGLIDADG- and 10 GIY-YIG-homing endonucleases. The nucleotide composition is characterized by adenine (A) at 37.02%, cytosine (C) at 12.91%, guanine (G) at 13.04%, and thymine (T) at 37.03%, culminating in a GC content of 25.95%. Subsequently, we undertook a phylogenetic analysis, employing a dataset of 25 mitochondrial genomes to construct a phylogenetic tree. This research represents the first comprehensive foray into understanding the phylogenetic relationships of T. fissilis with its Basidiomycete kin, particularly its sister-group relationship with Phlebia radiata Fr. (1821), thereby laying a substantive groundwork for subsequent evolutionary and taxonomic studies within this mycological cohort.