Protective effects of bone marrow mesenchymal stem cell-derived exosomes loaded cerium dioxide nanoparticle against deoxynivalenol-induced liver damage.

IF 12.6 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zitong Meng, Mingmeng Tang, Shiyin Xu, Xiaolei Zhou, Zixuan Zhang, Liunan Yang, Andreas K Nüssler, Liegang Liu, Wei Yang
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引用次数: 0

Abstract

Background: Deoxynivalenol (DON), a mycotoxin produced by Fusarium species, posed significant threats to food safety and human health due to its widespread prevalence and detrimental effects. Upon exposure, the liver, which played a crucial role in detoxifying DON, experienced depleted antioxidant levels and heightened inflammatory responses. Bone marrow mesenchymal stem cell (BMSC)-derived exosomes (BMSC-exos) exhibited therapeutic potential by promoting cellular repair and delivering bioactive substances, such as cerium dioxide nanoparticles (CeO₂ NPs), which are recognized for their ability to mitigate oxidative stress and inflammation.

Results: We successfully loaded BMSC-exos with CeO2 NPs (BMSC-exos @ CeO2) using extrusion techniques, verified through electron microscopy and elemental mapping. The resulting BMSC-exos @ CeO2 displayed low cytotoxicity, boosted antioxidant activity, and reduced inflammation in Hepa 1-6 cells with DON condition. In vivo study, BMSC-exos @ CeO2 maintained stability for 72 h, it also can prevent antioxidant depletion and inhibit liver inflammation under the DON condition. After BMSC-exos @ CeO2 treatment, multi-omics analyses further highlighted significant changes in metabolic and protein signaling pathways, notably in linoleic and arachidonic acid metabolism. Key pathways about AMPK and JAK1/STAT3 were involved in mitigating liver damage with or without DON.

Conclusion: Our findings revealed BMSC-exos @ CeO2 as a promising therapeutic strategy against DON's toxicity, offering valuable insights into their potential for liver protection.

负载二氧化铈纳米颗粒的骨髓间充质干细胞来源的外泌体对脱氧雪腐镰刀醇诱导的肝损伤的保护作用。
脱氧雪腐镰刀菌醇(脱氧雪腐镰刀菌醇,DON)是镰刀菌产生的一种真菌毒素,由于其广泛流行和有害影响,对食品安全和人类健康构成重大威胁。暴露后,在解毒DON中起关键作用的肝脏经历了抗氧化水平的下降和炎症反应的加剧。骨髓间充质干细胞(BMSC)衍生的外泌体(BMSC-exos)通过促进细胞修复和传递生物活性物质(如二氧化铈纳米颗粒(CeO₂NPs))显示出治疗潜力,二氧化铈纳米颗粒被认为具有减轻氧化应激和炎症的能力。结果:我们使用挤压技术成功地将CeO2 NPs (BMSC-exos @ CeO2)装入了BMSC-exos,并通过电子显微镜和元素图谱进行了验证。得到的BMSC-exos @ CeO2在DON条件下的Hepa 1-6细胞中表现出低细胞毒性,增强抗氧化活性,减少炎症。在体内研究中,BMSC-exos @ CeO2在DON条件下可维持72 h的稳定性,并能防止抗氧化剂消耗和抑制肝脏炎症。在BMSC-exos @ CeO2处理后,多组学分析进一步强调了代谢和蛋白质信号通路的显著变化,特别是亚油酸和花生四烯酸代谢。AMPK和JAK1/STAT3的关键通路参与减轻DON或不DON的肝损伤。结论:我们的研究结果表明BMSC-exos @ CeO2是一种很有前景的抗DON毒性治疗策略,为其肝脏保护潜力提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Nanobiotechnology
Journal of Nanobiotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
13.90
自引率
4.90%
发文量
493
审稿时长
16 weeks
期刊介绍: Journal of Nanobiotechnology is an open access peer-reviewed journal communicating scientific and technological advances in the fields of medicine and biology, with an emphasis in their interface with nanoscale sciences. The journal provides biomedical scientists and the international biotechnology business community with the latest developments in the growing field of Nanobiotechnology.
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