Caffeic Acid Alleviates Chronic Sleep Deprivation-Induced Intestinal Damage by Inhibiting the IMD Pathway in Drosophila.

IF 4.2 2区医学 Q2 IMMUNOLOGY

Journal of Inflammation Research Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI:10.2147/JIR.S500892

Dan Yang, Minghui Xiu, Xiaolin Jiang, Qian Kang, Jinyu Fu, Shihong Zhou, Yongqi Liu, Jianzheng He

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引用次数: 0

Abstract

Background: Sleep is vital for maintaining the health of the organism. Chronic sleep deprivation (CSD) is a key contributor to significant health risks, including the induction of gastrointestinal disorders. However, the mechanism of CSD caused intestinal damage remains unclear.

Methods: Drosophila melanogaster as an in vivo model was used to investigate the mechanism of CSD-induced intestinal injury, as well as the ameliorative effect of caffeic acid.

Results: CSD resulted in reduced survival and severely affected intestinal homeostasis in flies, as evidenced by disruption of intestinal acid-base homeostasis, increased feeding, increased intestinal permeability and shortened intestinal length. Meanwhile, the expressions of the immune deficiency (IMD) pathway-related genes PGRP-SB1, Dpt, AttA, AttB and Mtk were significantly up-regulated in the intestine of CSD flies. On the other hand, Caffeic acid supplementation restored intestinal acid-base homeostasis and intake, while improving intestinal barrier permeability and intestinal length, and effectively reducing intestinal damage. In addition, administration of caffeic acid decreased the expressions of PGRP-SB1, Dpt, AttA and Mtk genes in the CSD flies gut.

Discussion: These results suggested that CSD could disrupt gut homeostasis in adult flies by overactivating the IMD pathway, while Caffeic acid has an obvious protective role on the gut homeostasis.

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咖啡酸通过抑制果蝇IMD通路减轻慢性睡眠剥夺引起的肠道损伤。

背景：睡眠对维持机体健康至关重要。慢性睡眠剥夺（CSD）是造成重大健康风险的一个关键因素，包括诱发胃肠道疾病。然而，CSD引起肠道损伤的机制尚不清楚。方法：以黑腹果蝇为体内模型，研究csd诱导肠道损伤的机制，以及咖啡酸的改善作用。结果：CSD导致果蝇存活降低，肠道内平衡受到严重影响，表现为肠道酸碱平衡被破坏，摄食增加，肠道通透性增加，肠道长度缩短。与此同时，免疫缺陷（IMD）途径相关基因PGRP-SB1、Dpt、AttA、AttB和Mtk在CSD果蝇肠道中的表达显著上调。另一方面，补充咖啡酸恢复肠道酸碱平衡和摄入，同时改善肠道屏障通透性和肠道长度，有效减轻肠道损伤。此外，咖啡酸降低了CSD果蝇肠道中PGRP-SB1、Dpt、AttA和Mtk基因的表达。讨论：这些结果表明，CSD可能通过过度激活IMD途径破坏成年果蝇肠道内稳态，而咖啡酸对肠道内稳态具有明显的保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Inflammation Research Immunology and Microbiology-Immunology

CiteScore

6.10

自引率

2.20%

发文量

658

审稿时长

16 weeks

期刊介绍： An international, peer-reviewed, open access, online journal that welcomes laboratory and clinical findings on the molecular basis, cell biology and pharmacology of inflammation.