Multidrug-Resistance and Biofilm Formation among Acinetobacter baumannii Isolated from Clinical Specimens.

Q3 Medicine
Poonam Yadav, Shyam Kumar Mishra, Sreska Shrestha, Ranjit Sah, Junu Richhinbung Rai, Hari Prasad Kattel, Sangita Sharma, Mark Willcox
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引用次数: 0

Abstract

Background: Acinetobacter baumannii has emerged as a problematic pathogen due to its ability to become resistant to antibiotics and form biofilms. The aim of this study was to explore antibiotic resistance and biofilm formation, and examine any correlation between these in Acinetobacter baumannii isolates.

Methods: This was a cross-sectional study conducted at the 750-bed Tribhuvan University Teaching Hospital in Nepal. Identification and antibiotic sensitivity of Acinetobacter baumannii isolates were performed following American Society for Microbiology guidelines. Different β-lactamases were detected by standard phenotypic tests. The microtiter plate method was used to screen strains of their ability to form biofilms.  Results: Out of total 18,343 clinical samples processed, 4,249 (23.1%) showed bacterial growth. A. baumannii comprised of 4.7% of the total bacterial growth. Multidrug-resistant (MDR) was exhibited by 97.5% of Acinetobacter baumannii isolates. All multidrug-resistant Acinetobacter baumannii isolates were resistant to cephalosporins and carbapenems; however, they were sensitive to polymyxins. Only few isolates showed sensitivity to sulbactam-containing antibiotics (15.4-29.2%), fluoroquinolones (1.0-7.2%), aminoglycosides (2.6-5.6%), and cotrimoxazole (4.1%). Extended-spectrum-beta-lactamase (ESBL), metallo-beta-lactamase (MBL), Klebsiella pneumoniae carbapenemase (KPC) and AmpC production were found in 54.9%, 73.3%, 41.5% and 14.9% isolates, respectively. Among all tested isolates, 192 were able to produce biofilms, with 83.1% being classified as strong biofilm producers. Those strains that were resistant to gentamicin were more likely to produce biofilms (P<0.05). ESBL, MBL, KPC and AmpC were seen in 51.8%, 71.6%, 43.8% and 16.0% of strong biofilm producers respectively.

Conclusions:   Only polymyxins were effective against Acinetobacter baumannii. Carbapenemase producers were generally strong biofilm producers, and gentamicin resistant strains were more likely to produce biofilms. The findings of this study may help to understand antibiotic-resistance mechanisms and provide valuable information in the treatment of MDR Acinetobacter baumannii infections.

临床分离鲍曼不动杆菌的多药耐药及生物膜形成。
背景:鲍曼不动杆菌已经成为一种有问题的病原体,因为它能够对抗生素产生耐药性并形成生物膜。本研究的目的是探讨鲍曼不动杆菌分离株的抗生素耐药性和生物膜形成,并检查它们之间的任何相关性。方法:这是一项横断面研究,在尼泊尔有750个床位的特里布万大学教学医院进行。鲍曼不动杆菌分离株的鉴定和抗生素敏感性按照美国微生物学会的指南进行。通过标准表型试验检测不同的β-内酰胺酶。采用微滴板法筛选菌株形成生物膜的能力。结果:18343份临床样品中有4249份(23.1%)有细菌生长。鲍曼不动杆菌占细菌生长总量的4.7%。97.5%的鲍曼不动杆菌分离株具有多重耐药(MDR)。所有多重耐药鲍曼不动杆菌分离株均对头孢菌素和碳青霉烯类耐药;然而,它们对多粘菌素敏感。对含舒巴坦类抗生素(15.4 ~ 29.2%)、氟喹诺酮类(1.0 ~ 7.2%)、氨基糖苷类(2.6 ~ 5.6%)和复方新诺明(4.1%)敏感的菌株较少。广谱β -内酰胺酶(ESBL)、金属β -内酰胺酶(MBL)、肺炎克雷伯菌碳青霉烯酶(KPC)和AmpC的产率分别为54.9%、73.3%、41.5%和14.9%。在所有测试的分离株中,有192株能够产生生物膜,其中83.1%被归类为强生物膜产生者。对庆大霉素耐药的菌株更容易产生生物膜。结论:只有多粘菌素对鲍曼不动杆菌有效。碳青霉烯酶产生菌通常是强生物膜产生菌,而庆大霉素耐药菌株更容易产生生物膜。本研究结果可能有助于了解耐药机制,并为耐多药鲍曼不动杆菌感染的治疗提供有价值的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
81
审稿时长
15 weeks
期刊介绍: The journal publishes articles related to researches done in the field of biomedical sciences related to all the discipline of the medical sciences, medical education, public health, health care management, including ethical and social issues pertaining to health. The journal gives preference to clinically oriented studies over experimental and animal studies. The Journal would publish peer-reviewed original research papers, case reports, systematic reviews and meta-analysis. Editorial, Guest Editorial, Viewpoint and letter to the editor are solicited by the editorial board. Frequently Asked Questions (FAQ) regarding manuscript submission and processing at JNHRC.
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