Francisca Livia Parente Viana, Clara Edvirgens Oliveira Sousa, Ana Grasiela Limoeiro, Murilo Priori Alcalde, Rodrigo Ricci Vivan, Marco Antônio Hungaro Duarte, Ericka Tavares Pinheiro, Bruno Carvalho Vasconcelos
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引用次数: 0
Abstract
Background: The influence of ultrasonic agitation (UA) on the pH and antibiofilm activity of AH Plus Jet (AHP) and BioRoot RCS (BCS) sealers was evaluated. Subgroups were created based on the sealer/UA application.
Material and methods: The pH was measured at 30 min., 3, 24, 72, 168 h. Antibiofilm activity was determined by direct and indirect contact tests (DCT/ICT) on dual-species biofilms (Enterococcus faecalis and Streptococcus oralis). Bacterial survival was assessed by colony-forming unit (CFU) counting. The Mann-Whitney test was applied for th pH analysis whereas the Kruskall-Wallis and Dunn tests were employed for the DCT/ICT evaluations.
Results: BCS presented the highest pH at all time points (p<0.05). Related to UA, it significantly reduced the pH at 30 min., 3, 24 and 72 h (p<0.05). In the DCT, a significant reduction in CFUs was observed in the BCS and BCS/UA groups compared to the control and AHP/UA group. BCS also showed the best results in the ICT (p<0.05).
Conclusions: It was concluded that UA reduced pH and did not improve the sealers' antibiofilm activity. BCS showed the highest pH values and antibiofilm activity. Key words:Antibiofilm activity, Enterococcus faecalis, Streptococcus oralis, Root canal sealers, Ultrasonic activation.
期刊介绍:
Indexed in PUBMED, PubMed Central® (PMC) since 2012 and SCOPUSJournal of Clinical and Experimental Dentistry is an Open Access (free access on-line) - http://www.medicinaoral.com/odo/indice.htm. The aim of the Journal of Clinical and Experimental Dentistry is: - Periodontology - Community and Preventive Dentistry - Esthetic Dentistry - Biomaterials and Bioengineering in Dentistry - Operative Dentistry and Endodontics - Prosthetic Dentistry - Orthodontics - Oral Medicine and Pathology - Odontostomatology for the disabled or special patients - Oral Surgery