Characterization of the gut microbiota in urinary calculi patients with preoperative urinary tract infection.

IF 4.6 2区医学 Q2 IMMUNOLOGY

Frontiers in Cellular and Infection Microbiology Pub Date : 2025-02-28 eCollection Date: 2025-01-01 DOI:10.3389/fcimb.2025.1417403

Hao Chen, Jing Yuan, Hongmin Zhou, Xiangcheng Zhan, Yuchen Gao, Bowen Chen, Nuer Aihemaiti, Xiao Xu, Yunze Dong, Shuai Liu, Yanhua Chen, Ding Liu, Tiancheng Xie, Yunfei Xu

{"title":"Characterization of the gut microbiota in urinary calculi patients with preoperative urinary tract infection.","authors":"Hao Chen, Jing Yuan, Hongmin Zhou, Xiangcheng Zhan, Yuchen Gao, Bowen Chen, Nuer Aihemaiti, Xiao Xu, Yunze Dong, Shuai Liu, Yanhua Chen, Ding Liu, Tiancheng Xie, Yunfei Xu","doi":"10.3389/fcimb.2025.1417403","DOIUrl":null,"url":null,"abstract":"Background: Urinary tract infection is one of the most common comorbidities of urinary stones. Disorders of gut microbiota can affect various infectious diseases and the formation of the stones. Therefore, alterations in the gut bacteria profile may be a potential risk factor for the development of infections in patients with urinary tract stones.Methods: We conducted a retrospective study to analyze the association of urinary tract infections with gut microbiota and serum metabolism in patients with stones.Results: Patients with urolithiasis were predominantly in combination with diabetes mellitus (11.4% vs. 20%) and hypertension (36.4% vs. 50%). There were no statistically significant differences in hematological and urinary parameters. Compared to negative patients, IL-17A was significantly higher in the positive group (25.0 vs 21.1 pg/ml p = 0.038). The majority of pathogens detected in urine cultures were urease-negative bacteria, and urease-positive bacteria accounted for 15% of the total number of patients. We analyzed the community composition of the two groups of patients and found a significant difference in their β-diversity (p = 0.025), suggesting that dysbiosis of the gut bacteria may be associated with the combination of urinary tract infections in urolithiasis. For identification of crucial bacteria, we found changes in the abundance of both Intestinibacter (p = 0.036) and Dialister (p = 0.039), and abundance of Intestinibacter was positively correlated with IFN-α, IL-12P70 (p<0.05), and especially IL-17A (p<0.01), which may result from differences in translational, ribosomal structural and biosynthetic functions in stone patients (p < 0.05).Conclusion: Urolithiasis with gut dysbiosis developed a higher incidence of urinary tract infections, which may be associated with the increasing of Intestinibacter and affect the expression of IL-17A by translational, ribosomal structural and biosynthetic function.","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1417403"},"PeriodicalIF":4.6000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906712/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Cellular and Infection Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fcimb.2025.1417403","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Urinary tract infection is one of the most common comorbidities of urinary stones. Disorders of gut microbiota can affect various infectious diseases and the formation of the stones. Therefore, alterations in the gut bacteria profile may be a potential risk factor for the development of infections in patients with urinary tract stones.

Methods: We conducted a retrospective study to analyze the association of urinary tract infections with gut microbiota and serum metabolism in patients with stones.

Results: Patients with urolithiasis were predominantly in combination with diabetes mellitus (11.4% vs. 20%) and hypertension (36.4% vs. 50%). There were no statistically significant differences in hematological and urinary parameters. Compared to negative patients, IL-17A was significantly higher in the positive group (25.0 vs 21.1 pg/ml p = 0.038). The majority of pathogens detected in urine cultures were urease-negative bacteria, and urease-positive bacteria accounted for 15% of the total number of patients. We analyzed the community composition of the two groups of patients and found a significant difference in their β-diversity (p = 0.025), suggesting that dysbiosis of the gut bacteria may be associated with the combination of urinary tract infections in urolithiasis. For identification of crucial bacteria, we found changes in the abundance of both Intestinibacter (p = 0.036) and Dialister (p = 0.039), and abundance of Intestinibacter was positively correlated with IFN-α, IL-12P70 (p<0.05), and especially IL-17A (p<0.01), which may result from differences in translational, ribosomal structural and biosynthetic functions in stone patients (p < 0.05).

Conclusion: Urolithiasis with gut dysbiosis developed a higher incidence of urinary tract infections, which may be associated with the increasing of Intestinibacter and affect the expression of IL-17A by translational, ribosomal structural and biosynthetic function.

查看原文本刊更多论文

术前尿路感染尿路结石患者肠道菌群特征分析。

背景：尿路感染是泌尿系结石最常见的合并症之一。肠道菌群紊乱会影响各种传染病和结石的形成。因此，肠道细菌谱的改变可能是尿路结石患者发生感染的潜在危险因素。方法：我们进行了一项回顾性研究，分析尿路感染与结石患者肠道微生物群和血清代谢的关系。结果：尿石症患者以合并糖尿病（11.4%比20%）和高血压（36.4%比50%）为主。两组在血液学和泌尿学指标上无统计学差异。与阴性患者相比，阳性组IL-17A显著升高（25.0 vs 21.1 pg/ml p = 0.038）。尿培养中检出的病原菌以脲酶阴性菌居多，脲酶阳性菌占患者总数的15%。我们分析了两组患者的群落组成，发现其β-多样性存在显著差异（p = 0.025），提示肠道菌群失调可能与尿石症患者合并尿路感染有关。对于关键细菌的鉴定，我们发现了testinibacter （p = 0.036）和Dialister （p = 0.039）丰度的变化，并且testinibacter丰度与IFN-α、IL-12P70正相关(p结论：尿石症合并肠道生态失调患者尿路感染发生率较高，这可能与testinibacter的增加有关，并可能通过翻译、核糖体结构和生物合成功能影响IL-17A的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Frontiers in Cellular and Infection Microbiology IMMUNOLOGY-MICROBIOLOGY

CiteScore

7.90

自引率

7.00%

发文量

1817

审稿时长

14 weeks

期刊介绍： Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.