Comparative phenotypic, physiological, and transcriptomic responses to drought and recovery in two Fraxinus species.

Abstract

Background: This study focused on the drought tolerance and resilience of two ash species: Fraxinus chiisanensis and F. rhynchophylla. These two species are distributed in different habitats, suggesting that they have different levels of drought tolerance. Understanding their response to drought stress, particularly during the seedling stage, is crucial for selecting and developing drought-resistant varieties. This study aimed to compare the phenotypic, physiological, and transcriptomic characteristics of drought-stressed and recovered rewatered plants in a time-course experiment.

Results: In F. rhynchophylla, drought stress resulted in more severe growth retardation, temperature increase, and a faster decline in the fluorescence response, accompanied by a significant rise in stress indices. However, these reactions recovered quickly after rehydration. In contrast, F. chiisanensis exhibited less growth retardation, a slower decline in fluorescence, and milder increases in stress indices, although many individuals did not fully recover after rehydration. The activity of antioxidant enzymes (SOD, CAT, APX) was more responsive and recovered more efficiently in F. rhynchophylla, while F. chiisanensis had a weaker and delayed response. Transcriptome analysis revealed that photosynthesis and enzyme activity were the most responsive to drought and recovery, as shown by Gene Ontology term analysis. Kyoto Encyclopedia of Genes and Genomes pathway analysis identified common pathways involved in starch and sucrose metabolism and phenylpropanoid biosynthesis in both species. F. rhynchophylla had more differentially expressed genes (DEGs) than F. chiisanensis, particularly on the drought and recovery day 6. Most drought-induced DEGs were restored after rehydration. Commonly associated genes included BGLU and TPS in sugar metabolism; CAT, GSTF, TT7, and HCT in antioxidant enzymes; PYL4 and RR17 in hormone signaling; and ADC1 and ASP3 in proline synthesis.

Conclusions: This study highlights the species-specific characteristics of drought and recovery responses of two Fraxinus species and provides targets for assessing and improving drought tolerance. Moreover, the results of this study provide insights into the physiological and genetic responses of Fraxinus and may guide future research on ash tree stress tolerance.