Short-term sustained hypoxia distinctly affects subpopulations of carotid body glomus cells from rats.

IF 5 2区 生物学 Q2 CELL BIOLOGY
Pedro F Spiller, Henrique J N Morgan, Luiz C C Navegantes, Benedito H Machado, Melina P da Silva, Davi J A Moraes
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引用次数: 0

Abstract

The main O2 arterial chemoreceptors are the carotid bodies (CBs), which mediate hyperventilation in response to short-term sustained hypoxia (SH). CBs contain glomus cells expressing K+ channels, which are inhibited by hypoxia, leading to neurotransmitter release. ATP released by CBs and type II cells has been considered essential for chemosensory processing under physiological and pathophysiological conditions. Although the systemic effects of chronic activation of CBs by SH are well known, the early (first 24 h) cellular and molecular mechanisms in CBs as well as the effects of short-term SH on populations of glomus cells are still poorly understood. Here, we show that SH (10% O2 for 24 h) depolarizes the membrane potential of one population of glomus cells, mediated by increases in inward current, but does not affect the ATP release by CBs. In addition, SH promotes a reduction in their maximum outward current, mediated by voltage-gated K+ channels. SH also affected sensitivity to acute hypoxia in one glomus cell subpopulation. As for the content of mitochondrial proteins, we observed increases in the citrate synthase, Tom-20, and succinate dehydrogenase (mitochondrial complex II) per cell of CBs after SH. Our results demonstrate important cellular and molecular mechanisms of plasticity in CBs from rats after only 24 h of SH, which may contribute to the generation of cardiovascular and ventilatory adjustments observed in this experimental model.NEW & NOTEWORTHY Our study revealed two subpopulations of glomus cells of carotid bodies (CBs) with specific electrophysiological properties, which were differentially affected by short-term sustained hypoxia (SH; 10% O2 for 24 h). Our experiments showed that SH also affected the sensitivity to acute hypoxia of these glomus cell subpopulations differently. Our molecular analyses allowed us to identify important adaptations in the content of CB mitochondrial proteins that participate in the Krebs cycle and form the electron transport chain.

短期持续缺氧明显影响大鼠颈动脉体血管球细胞亚群。
主要的氧动脉化学感受器是颈动脉小体(CBs),它在短期持续缺氧(SH)时介导过度通气。CBs含有表达K+通道的血管球细胞,该通道被缺氧抑制,导致神经递质释放。CBs球囊细胞和II型细胞释放的ATP被认为是生理和病理生理条件下化学感觉加工的必要条件。虽然SH对CBs慢性激活的全身性影响是众所周知的,但CBs的早期(最初24小时)细胞和分子机制(最初24小时)以及短期SH对球囊细胞群的影响仍然知之甚少。在此,我们表明SH (10% O2 24小时)通过增加向内电流介导使一群血管球细胞的膜电位去极化,但不影响CBs释放ATP。此外,SH通过电压门控K+通道介导,促进其最大外向电流的降低。SH对两种球囊细胞亚群的急性缺氧敏感性也有不同的影响。至于线粒体蛋白的含量,我们观察到SH后CBs每个细胞的柠檬酸合成酶、汤姆-20和琥珀酸脱氢酶(线粒体复合体II)的增加。我们的研究结果证明了SH仅24小时后大鼠CBs可塑性的重要细胞和分子机制,这可能有助于本实验模型中观察到的心血管和通气调节的产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
9.10
自引率
1.80%
发文量
252
审稿时长
1 months
期刊介绍: The American Journal of Physiology-Cell Physiology is dedicated to innovative approaches to the study of cell and molecular physiology. Contributions that use cellular and molecular approaches to shed light on mechanisms of physiological control at higher levels of organization also appear regularly. Manuscripts dealing with the structure and function of cell membranes, contractile systems, cellular organelles, and membrane channels, transporters, and pumps are encouraged. Studies dealing with integrated regulation of cellular function, including mechanisms of signal transduction, development, gene expression, cell-to-cell interactions, and the cell physiology of pathophysiological states, are also eagerly sought. Interdisciplinary studies that apply the approaches of biochemistry, biophysics, molecular biology, morphology, and immunology to the determination of new principles in cell physiology are especially welcome.
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