Amelioration of Glutamate-induced Toxicity by a New Thyrotropin-releasing Hormone (TRH) Analogue PYR-l-(2,5-Dibromo)-His-l-ProNH₂.

IF 1.8 Q4 NEUROSCIENCES

Annals of Neurosciences Pub Date : 2025-03-13 DOI:10.1177/09727531241305505

Mallikarjuna R Sunkara, Jitendra N Singh, C L Meena, A B Pant, Rahul Jain, Shyam S Sharma

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Abstract

Background: Glutamate has been implicated in the pathophysiology of central nervous system diseases, including stroke.

Purpose: In this study, the neuroprotective potential of the newly synthesised thyrotropin-releasing hormone (TRH) analogue [Pyr-l-(2,5-dibromo)-His-l-ProNH₂; NP-2376] against glutamate-induced injury model were investigated.

Methods: Cortical neurons isolated from neonatal rats were used to evaluate the effects of the NP-2376. Cortical neurons were pre-treated with NP-2376 (6, 12 and 24 h) prior to glutamate (15 mM) exposure. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assay, and oxidative stress by chloromethyl-2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) and glutathione assays.

Results: NP-2376 protected against glutamate-induced cortical neuron death and oxidative stress in a dose-dependent manner.

Conclusions: This study demonstrates the neuroprotective potential of TRH analogue NP-2376 against glutamate-induced toxicity, which is attributed to a decrease in oxidative stress.

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一种新的促甲状腺激素释放激素（TRH）类似物PYR-l-(2,5-二溴)-His-l-ProNH2可改善谷氨酸诱导的毒性。

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