Historical Lead Contamination Linked to Atmospheric Deposition is Associated With Declines in Ectomycorrhizal Diversity and Shifts in Fungal Community Composition

Abstract

Ectomycorrhizal and saprotrophic fungi respond differently to changing edaphic conditions caused by atmospheric deposition. Within each guild, responses can vary significantly, reflecting the diversity of species and their specific adaptations to environmental changes. Metal contaminants are often deposited onto earth's surface through atmospheric deposition, yet few studies have assessed the relationship between soil metal contamination and fungal communities. The goal of this study was to understand how soil metal contamination and other edaphic factors vary across the spruce-fir ecosystem in the Southern Appalachians and influence fungal diversity and function. Here, we characterize soil fungal communities using high-throughput sequencing of the ITS2 gene region and found that higher soil lead (Pb) concentrations were associated with lower fungal diversity. Ectomycorrhizal fungi were less diverse (specifically hydrophilic ectomycorrhizal functional types) at plots with elevated soil Pb concentrations, while saprotrophic fungi were less diverse at plots with elevated soil carbon:nitrogen ratios. Fungal community composition was significantly influenced by pH, Pb, and spatial factors. This study identifies important relationships between fungal diversity and soil Pb concentrations and indicates variable responses of genera within well-defined ecological guilds. Our work highlights the need to characterize poorly understood taxonomic groups of fungi and their function prior to further environmental degradation.