Xiaoshan Luo, Liudi Liu, Xuanbo Wang, Disi Lu, Yuexi Liang, Osmani Chacón Chacón, Gang Li, Xiaoqin Wang and Zhaozhu Zheng
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引用次数: 0
Abstract
In this study, we present a facile and efficient method for fabricating silk fibroin hydrogel films encapsulating the acetylcholinesterase enzyme (AChE) and pH test strips (biosensor strips) for detecting organophosphorus pesticides (OPs) and aflatoxin B1 (AFB1). These biosensor strips possess several unique properties: flexibility, time efficiency, and high stability. Upon exposure to OPs or AFB1, AChE activity is inhibited, leading to a discernible color change on the pH test strip, enabling rapid and sensitive visual detection of these contaminants. The strips were effectively utilized as a practical and sensitive platform for the visual detection of OPs and AFB1. The limits of detection for paraoxon, aldicarb, and AFB1 were determined to be 6.57, 8.92, and 0.274 ng mL−1, respectively. Furthermore, the applicability of these biosensors for detecting OPs or AFB1 in real samples of Chinese cabbage and peanuts was successfully demonstrated. Additionally, the performance of these novel strip composites was validated through comparison with commercial pesticide rapid test papers. Notably, the encapsulated AChE enzymes retained significant sensitivity for over 18 months, even when stored at 37 °C, and the biosensor strips exhibited remarkable resistance to sensitivity loss caused by inhibitors. This work presents a highly sensitive and stable enzyme-based visual detection paper for agricultural and food safety applications. In addition to its outstanding resistance to interference, the developed biosensor ensures reliable reproducibility and maintains stability over long-term use. This new approach to OP analysis in ecological samples has also been effectively used in the determination of OPs in lake water.
在本研究中,我们提出了一种简单有效的方法来制备包裹乙酰胆碱酯酶(AChE)的丝素蛋白水凝胶膜和用于检测有机磷农药(OPs)和黄曲霉毒素B1 (AFB1)的pH试纸条(生物传感器试纸条)。这些生物传感器条具有几个独特的特性:灵活性,时间效率和高稳定性。暴露于OPs或AFB1后,AChE活性被抑制,导致pH试纸上出现明显的颜色变化,从而实现对这些污染物的快速、灵敏的视觉检测。该试纸条可作为一种实用、灵敏的OPs和AFB1视觉检测平台。对氧磷、灭威和AFB1的检出限分别为6.57、8.92和0.274 ng mL-1。此外,成功地证明了这些生物传感器在白菜和花生实际样品中检测OPs或AFB1的适用性。此外,通过与市售农药快速试纸的对比,验证了新型条状复合材料的性能。值得注意的是,包封的AChE酶即使在37°C下保存,也能在18个月以上保持显著的敏感性,并且生物传感器条对抑制剂引起的敏感性损失表现出显著的抵抗力。本工作提出了一种高灵敏度和稳定的酶基视觉检测纸,用于农业和食品安全应用。除了具有出色的抗干扰能力外,开发的生物传感器还可确保可靠的再现性,并在长期使用中保持稳定性。生态样品中有机磷分析的新方法也已有效地应用于湖泊水体中有机磷的测定。